Castasterone Can be Biosynthesized from 28-homodolichosterone in Arabidopsis thaliana
- Authors
- Kim, Sunyoung; Moon, Jinyoung; Roh, Jeehee; Kim, Seong-Ki
- Issue Date
- Oct-2018
- Publisher
- SPRINGER HEIDELBERG
- Keywords
- Arabidopsis thaliana; Biosynthesis of 24-ethylidene brassinosteroids; Biosynthetic connection of C-28-Brassinosteroids and C-29-brassinosteroids; Metabolism of 28-homodolichosterone; Synthesis of castasterone
- Citation
- JOURNAL OF PLANT BIOLOGY, v.61, no.5, pp 330 - 335
- Pages
- 6
- Journal Title
- JOURNAL OF PLANT BIOLOGY
- Volume
- 61
- Number
- 5
- Start Page
- 330
- End Page
- 335
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/721
- DOI
- 10.1007/s12374-018-0194-4
- ISSN
- 1226-9239
1867-0725
- Abstract
- We recently demonstrated the biosynthesis of 24-ethylidene brassinosteroids in Arabidopsis thaliana. To determine the physiological role of biosynthesis of 24-ethylidene brassinosteroids, metabolism of 28-homodolichosterone as the end product of 24-ethylidene brassinosteroids biosynthesis was examined by a crude enzyme solution prepared from A. thaliana. In wild-type plants, dolichosterone and castasterone were identified as enzyme products on GC-MS analysis. In a mutant where DWARF1 was overexpressed (35S-DWF1), the conversion rate of 28-homodolichosterone to castasterone was significantly increased. These results indicate that conversion of 28-homodolichosterone to castasterone is mediated by dolichosterone in Arabidopsis. In the root growth assay, inhibitory activity was enhanced in the order of castasterone > dolichosterone > 28-homodolichosterone, demonstrating that conversion of 28-homodolichosterone to castasterone via dolichosterone is a biosynthetic reaction that increases BR activity in Arabidopsis. Compared to Arabidopsis grown under dark conditions, light-grown Arabidopsis showed up-regulated DWARF1 expression, resulting in an increased conversion rate of 28-homodolichosterone to castasterone, suggesting that light is an important regulatory factor for the biosynthetic connection of 24-ethylidene brassinosteroids and 24-methyl brassinosteroids in A. thaliana. Consequently, 24-ethylidene brassinosteroids biosynthesis to generate 28-homodolichosterone is a lightregulated alternative route for synthesis of the biologically-active BRs, castasterone and brassinolide in Arabidopsis plants.
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Collections - College of Natural Sciences > Department of Life Science > 1. Journal Articles
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