Interactions of dendritic cells with cancer cells and modulation of surface molecules affect functional properties of CD8<SUP>+</SUP> T cells
- Authors
- Seo, Min Ji; Kim, Gi Rak; Yang, Deok-Chun; Chu, Hyuk; Min, Tae Sun; Son, Young Min; Jung, In Duk; Park, Yeong-Min; Han, Seung Hyun; Yun, Cheol-Heui
- Issue Date
- Sep-2011
- Publisher
- PERGAMON-ELSEVIER SCIENCE LTD
- Keywords
- Dendritic cells; Cancer cells; Major histocompatibility complex; CD8(+) T cells; Antigen processing component
- Citation
- MOLECULAR IMMUNOLOGY, v.48, no.15-16, pp 1744 - 1752
- Pages
- 9
- Journal Title
- MOLECULAR IMMUNOLOGY
- Volume
- 48
- Number
- 15-16
- Start Page
- 1744
- End Page
- 1752
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/72126
- DOI
- 10.1016/j.molimm.2011.04.018
- ISSN
- 0161-5890
- Abstract
- To understand the interaction of dendritic cells (DCs) with cancer cells, we investigated molecular changes in DCs following co-culture with cancer cells. DCs co-cultured with Jurkat cancer cells showed remarkable down-regulation of MHC class I molecules, while DCs co-cultured with MCF-7 cancer cells showed minimal changes. Interestingly, down-regulation of MHC class Ion DCs was not observed upon treatment with Jurkat cell lysate or culture supernatant, suggesting the importance of direct cell-cell interactions. The expressions of CD40, CD80, CD83, MHC class II, and IL-12p40 on DCs co-cultured with Jurkat cells were only slightly affected. In contrast, DCs co-cultured with MCF-7 cells showed increased expressions of CD80, CD83, CD86, and IL-12p40. Furthermore, DCs co-cultured with Jurkat cells showed a down-regulation of low molecular weight polypeptides (LMP) 7, and of transporter associated with antigen processing (TAP) 1 and 2 at the mRNA expression level. LMP7, TAP2 and beta 2-microglobulin (beta 2M) were also down-regulated at the protein level. We further demonstrated how altered expression of MHC class I on DCs caused by co-culture with cancer cells affected autologous CD8(+) T cells, using the model MHC class l-presented HSV antigen. We found that DCs that had been HSV-treated and co-cultured with Jurkat cells showed a reduced potency to activate CD8(+) T cells. In contrast, HSV-treated DCs that had been co-cultured with MCF-7 cells induced activation of CD8(+) T cells, including high expression of CD25, CD69, granzyme B and cytokines, TNF-alpha and IFN-gamma. (C) 2011 Elsevier Ltd. All rights reserved.
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Collections - College of Biotechnology & Natural Resource > Department of Systems Biotechnology > 1. Journal Articles
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