Nanozyme-assisted molecularly imprinted polymer-based indirect competitive ELISA for the detection of marine biotoxin
- Authors
- Cho, Chae Hwan; Kim, Ji Hong; Padalkar, Navnath S; Reddy, Y Veera Manohara; Park, Tae Jung; Park, Jinyoung; Park, Jong Pil
- Issue Date
- Jul-2024
- Publisher
- Elsevier Ltd
- Keywords
- Affinity peptide; ELISA; Marine toxin; Molecularly imprinted polymer; MIP; Nanozyme
- Citation
- Biosensors & bioelectronics, v.255
- Journal Title
- Biosensors & bioelectronics
- Volume
- 255
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/73326
- DOI
- 10.1016/j.bios.2024.116269
- ISSN
- 0956-5663
1873-4235
- Abstract
- Saxitoxin (STX), which is produced by certain dinoflagellate species, is a type of paralytic shellfish poisoning toxin that poses a serious threat to human health and the environment. Therefore, developing a technology for the convenient and cost-effective detection of STX is imperative. In this study, we developed an affinity peptide-imprinted polymer-based indirect competitive ELISA (ic-ELISA) without using enzyme-toxin conjugates. AuNP/Co3O4@Mg/Al cLDH was synthesized by calcining AuNP/ZIF-67@Mg/Al LDH, which was obtained by combining AuNPs, ZIF-67, and flower-like Mg/Al LDH. This synthesized nanozyme exhibited high catalytic activity (Km=0.24 mM for TMB and 132.5 mM for H2O2). The affinity peptide-imprinted polymer (MIP) was imprinted with an STX-specific template peptide (STX MIP) on a multi-well microplate and then reacted with an STX-specific signal peptide (STX SP). The interaction between the STX SP and MIP was detected using a streptavidin-coated nanozyme (SA-AuNP/Co3O4@Mg/Al cLDH). The developed MIP-based ic-ELISA exhibited excellent selectivity and sensitivity, with a limit of detection of 3.17 ng/mL (equivalent: 0.317 μg/g). Furthermore, the system was validated using a commercial ELISA kit and mussel tissue samples, and it demonstrated a high STX recovery with a low coefficient of variation. These results imply that the developed ic-ELISA can be used to detect STX in real samples. Copyright © 2024 Elsevier B.V. All rights reserved.
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