Profiling the Phytochemicals of <i>Orostachys margaritifolia</i>: Biological Activities, LC-ESI/MS, and HPLC Analysesopen access
- Authors
- Yoon, Nari; Yu, Hyeonjun; Tran, Gia Han; Ko, Chung Ho; Kim, Hoon; Yoon, Mi Jeong; Lee, Sanghyun
- Issue Date
- Mar-2024
- Publisher
- MDPI
- Keywords
- ABTS(+)-DPPH radical scavenging activity; HPLC/PDA; LC-ESI/MS; nitric oxide; Orostachys margaritifolia
- Citation
- PHARMACEUTICALS, v.17, no.3
- Journal Title
- PHARMACEUTICALS
- Volume
- 17
- Number
- 3
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/73349
- DOI
- 10.3390/ph17030290
- ISSN
- 1424-8247
1424-8247
- Abstract
- Orostachys margaritifolia Y. N. Lee (OMY) is an endemic Korean plant in the family Crassulaceae that is known to contain a variety of bioactive compounds. To assess the physiological activities of an OMY ethanol extract, ABTS+ and DPPH radical scavenging assays and a nitric oxide (NO) inhibition assay were conducted. The phytochemical makeup of the extract was profiled via liquid chromatography-mass spectrometry (LC-ESI/MS) and high-performance liquid chromatography with a photodiode array detector (HPLC/PDA). The OMY extract was found to have weaker ABTS+ and DPPH radical scavenging activities than the control group (green tea). In the NO inhibition assay, the OMY extract induced a significant increase in macrophage cell viability but showed a lower NO inhibitory activity than l-NAME, producing an IC50 value of 202.6 mu g/mL. The LC-ESI/MS and HPLC/PDA analyses identified isoquercitrin and astragalin in the OMY extract, quantifying their contents at 3.74 mg/g and 3.19 mg/g, respectively. The study revealed possibilities for the utilization of OMY as a future source of drugs for alleviating inflammation and diseases related to reactive oxygen species.
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