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High-yield methods for purification of alpha-linolenic acid from Perilla frutescens var. japonica oil
- Authors
- Lee, Jaemin; Lee, Myung Hee; Cho, Eun Ju; Lee, Sanghyun
- Issue Date
- Feb-2016
- Publisher
- KOREAN SOC APPLIED BIOLOGICAL CHEMISTRY
- Keywords
- GC-FID; Linolenic acid; Perilla frutescens var. japonica; Urea
- Citation
- APPLIED BIOLOGICAL CHEMISTRY, v.59, no.1, pp 89 - 94
- Pages
- 6
- Journal Title
- APPLIED BIOLOGICAL CHEMISTRY
- Volume
- 59
- Number
- 1
- Start Page
- 89
- End Page
- 94
- ISSN
- 2468-0834
2468-0842
- Abstract
- This study was conducted to investigate the purification methods for high-yield of alpha-linolenic acid (ALA) from Perilla frutescens var. japonica oil (PO). PO was treated with 2 g of urea, cooled for 24 h, and 81.75 % of ALA was obtained after gas chromatography-flame ionization detector analysis. Urea complexation at low temperature (refrigeration) is a prospective method for the extraction of unsaturated fatty acids in high yields. Our results suggest that urea treatment with cooling at 10 degrees C could be used for purification of ALA from PO. It will be enhanced for the development of nutritional, medical, and cosmetic value.
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