Utilization of chicory roots for microbial endoinulinase production

Abstract

Aims: The optimal culture conditions for endoinulinase production using chicory roots were studied in shake-flask culture. Methods and Results: Much higher enzyme production was achieved with Xanthomonas sp. (15 U ml-1) than with Pseudomonas sp. (3 U ml-1). Optimized culture conditions of Xanthomonas sp. for endoinulinase production in flask culture were: chicory powder, 5 g l-1; temperature, 37°C; pH, 7.0; agitation speed, 100 rev min-1. Conclusions: Maximum bacterial growth and enzyme production were 6.2 g l-1 and 20 U ml-1 under optimal conditions, respectively. Significance and Impact of the Study: Chicory roots could be used as a fermentation substrate for the production of enndoinulinase.