잔대 추출물과 이들 분획물들의 항산화 및 뇌신경세포 보호 효과Antioxidative and neuroprotective effects of extract and fractions from Adenophora triphylla
- Authors
- Chung, M.J.; Lee, S.; Park, Y.I.; Kwon, K.H.
- Issue Date
- 2016
- Publisher
- Korean Society of Food Science and Nutrition
- Keywords
- Adenophora triphylla; Antioxidant effect; Neuroprotective effects; Oxidative stress; SK-N-SH cells
- Citation
- Journal of the Korean Society of Food Science and Nutrition, v.45, no.11, pp 1580 - 1588
- Pages
- 9
- Journal Title
- Journal of the Korean Society of Food Science and Nutrition
- Volume
- 45
- Number
- 11
- Start Page
- 1580
- End Page
- 1588
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/8577
- DOI
- 10.3746/jkfn.2016.45.11.1580
- ISSN
- 1226-3311
2288-5978
- Abstract
- 잔대의 70% 에탄올 추출물은 강한 항산화 효과 및 산화적 스트레스 조건에서 뇌신경세포 사멸 억제 효과가 있었다. 6개의 분획물 중에 잔대 추출물의 클로로포름 분획물(ATCH)은 높은 DPPH 라디칼 소거작용 및 세포 내 활성산소종(ROS) 소거작용을 가지고 있었고 H₂O₂에 대항하여 뇌신경세포(SK-N-SH) 보호 효과가 가장 뛰어났다. 활성이 가장 우수한 클로로포름 분획물을 대상으로 주요 물질을 순수분리한 후 ¹H-NMR, 13C-NMR 그리고 EI mass spectra를 이용하여 화학구조를 분석한 결과 phytosterols(β-sitosterol과 daucosterol)이라는 것을 알 수 있었다. β-Sitosterol과 daucosterol 역시 산화적 스트레스에 대항하여 SK-NSH세포를 보호하는 작용이 있었다. AT-CH 그리고 이것으로부터 분리된 화합물은 p38 경로를 저해하였다. 이들 결과는 AT-CH는 p38 경로를 저해하고 세포 내 ROS 소거작용에 의해 산화적 스트레스로부터 뇌신경세포 보호 효과를 가질 것이라 제안하였다.
The 70% ethanol extract from Adenophora triphylla showed a strong antioxidant effect and reduced cytotoxicity of H2O2 in SK-N-SH cells. The chloroform fraction from A. triphylla extract (AT-CH) among the six fractions showed strong DPPH radical and intracellular reactive oxygen species (ROS) scavenger effects and the highest protective effect against H2O2-induced SK-N-SH cell death. Bioactivity compounds were purified from AT-CH, and the chemical structures of the compounds were determined as β-sitosterol and daucosterol on the basis of 1H-NMR, 13C-NMR, and EI mass spectra. β-Sitosterol and daucosterol also had protective effects against oxidative stress in SK-N-SH cells. Phospho-p38 MAPK levels were elevated by H2O2 but were inhibited by treatment with AT-CH and phytosterols (β-sitosterol and daucosterol) isolated from AT-CH. These results suggest that AT-CH has brain neuroprotective effects against oxidative stress (H2O2) by inhibiting activation of p38 pathways and scavenging intracellular ROS.
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