Colorimetric detection of pathogenic bacteria using platinum-coated magnetic nanoparticle clusters and magnetophoretic chromatography
- Authors
- Kwon, Donghoon; Lee, Sanghee; Ahn, Myung Mo; Kang, In Seok; Park, Ki-Hwan; Jeon, Sangmin
- Issue Date
- Jul-2015
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Magnetophoretic chromatography; Pathogenic bacteria; Platinum nanoparticles; Colorimetric detection
- Citation
- ANALYTICA CHIMICA ACTA, v.883, pp 61 - 66
- Pages
- 6
- Journal Title
- ANALYTICA CHIMICA ACTA
- Volume
- 883
- Start Page
- 61
- End Page
- 66
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9349
- DOI
- 10.1016/j.aca.2015.04.044
- ISSN
- 0003-2670
1873-4324
- Abstract
- A colorimetric method that uses platinum-coated magnetic nanoparticle clusters (Pt/MNCs) and magnetophoretic chromatography is developed to detect pathogenic bacteria. Half-fragments of monoclonal Escherichia coli O157:H7 (EC) antibodies were functionalized to Pt/MNCs and used to capture Escherichia coli bacteria in milk. After magnetic separation of free Pt/MNCs and Pt/MNC-EC complexes from the milk, a precision pipette was used to imbibe the Escherichia coli-containing solution, then a viscous polyethylene glycol solution. Due to difference in viscosities, the solutions separate into two liquid layers inside the pipette tip. The Pt/MNC-EC complexes were separated from the free Pt/MNCs by applying an external magnetic field, then added to a tetramethylbenzidine (TMB) solution. Catalytic oxidation of TMB by Pt produced color changes of the solution, which enabled identification of the presence of 10 cfu mL(-1) Escherichia coli bacteria with the naked eye. The total assay time including separation, binding and detection was 30 min. (C) 2015 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Biotechnology & Natural Resource > School of Food Science and Technology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9349)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.