Deglycosylation of isoflavone C-glycosides by newly isolated human intestinal bacteria
- Authors
- Kim, Mihyang; Lee, Jaekwan; Han, Jaehong
- Issue Date
- Jul-2015
- Publisher
- WILEY-BLACKWELL
- Keywords
- deglycosylation; human intestinal bacteria; isoflavone C-glycoside; isoflavone O-glycoside; puerarin
- Citation
- JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, v.95, no.9, pp 1925 - 1931
- Pages
- 7
- Journal Title
- JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
- Volume
- 95
- Number
- 9
- Start Page
- 1925
- End Page
- 1931
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9370
- DOI
- 10.1002/jsfa.6900
- ISSN
- 0022-5142
1097-0010
- Abstract
- BACKGROUNDPlant isoflavones are mostly present in the glycoside form. Isoflavone aglycones produced by intestinal microflora are reported to be more bioactive than the glycoside form. However, the deglycosylation of isoflavone C-glycosides is known to be rare, and is less studied. RESULTSThree new bacteria were isolated from human faecal samples, two of which hydrolysed the C-glycosidic bond of puerarin, daidzein-8-C-glucoside. They were identified as two Lactococcus species, herein designated as MRG-IFC-1 and MRG-IFC-3, and an Enterococcus species, herein designated MRG-IFC-2, based on their 16S rDNA sequences. From a reactivity study, it was found that Lactococcus sp. MRG-IFC-1 and Enterococcus sp. MRG-IFC-2 hydrolysed isoflavone C- and O-glycosides, as well as the flavone O-glycoside apigetrin, but could not hydrolyse the flavone C-glycosidic bond of vitexin. The other Lactococcus sp., MRG-IF-3, could not hydrolyse the C-glycosidic linkage of puerarin, while it showed a broad substrate spectrum of O-glycosidase activity similar to the other two bacteria. Puerarin was completely converted to daidzein within 100min by Lactococcus sp. MRG-IFC-1 and Enterococcus sp. MRG-IFC-2, which is the fastest conversion among the reported human intestinal bacteria. CONCLUSIONTwo new puerarin-metabolising human intestinal bacteria were isolated and identified, and the deglycosylation activity for various flavonoid glycosides was investigated. The results could facilitate the study of C-glycosidase reaction mechanisms, as well as the pharmacokinetics of bioactive C-glycoside natural products. (c) 2014 Society of Chemical Industry
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