Sequence-characterized amplified polymorphism markers for selecting rind stripe pattern in watermelon (Citrullus lanatus L.)
- Authors
- Kim, Hyeogjun; Han, Dongyeop; Kang, Jumsoon; Choi, Youngwhan; Levi, Amnon; Lee, Gung Pyo; Park, Younghoon
- Issue Date
- Jun-2015
- Publisher
- KOREAN SOC HORTICULTURAL SCIENCE
- Keywords
- cucurbits; DNA marker; fruit-related trait; marker-assisted breeding; maker-assisted selection
- Citation
- HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, v.56, no.3, pp 341 - 349
- Pages
- 9
- Journal Title
- HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
- Volume
- 56
- Number
- 3
- Start Page
- 341
- End Page
- 349
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/9526
- DOI
- 10.1007/s13580-015-0017-1
- ISSN
- 2211-3452
2211-3460
- Abstract
- The inheritance of foreground stripe pattern in rind of watermelon fruits [Citrullus lanatus (Thunb.) Matsum. & Nakai] was evaluated and molecular markers for selecting the Jubilee-type (JT) stripe pattern were developed based on bulked segregant analysis (BSA). Divergence in rind pattern among F-2 progeny derived from crossing Crimson-type (CT) 'Arka Manik' (AM) with JT 'TS34' (TS) indicated that stripe pattern is a quantitative trait controlled by more than one gene. The BSA of F-2 plants (derived from a cross between 'AM' and 'TS') using 60 random amplified polymorphic DNA (RAPD) primers revealed a distinct RAPD band (AT14-900) polymorphic between 'AM' (CT) and 'TS' (JT). The AT14-900 sequence (925 bp) was blasted to the reference watermelon (97103) genome and high sequence similarity (97.8%) was identified on physical location of 26246077 to 26246993 bp on chromosome 6. Two expressed sequence tags (ESTs) designated 'wsbin6-10' and 'wsbin6-11' that were closely linked to AT14-900 on a genetic linkage map (developed using the F-2 population derived from 'AM' x 'TS') were positioned 2,216 kb and 71 kb from AT14-900, respectively on the reference watermelon genome sequence. Marker genotyping of the F-2 population showed that wsbin6-11 was tightly linked to the JT stripe pattern of 'TS' and could be a useful codominant marker for selecting this trait. In a test using 100 breeding lines, 34 of the 36 lines carrying the JT stripe pattern were homozygous for the wsbin6-11 marker (450 bp) derived from 'TS', while other lines (e.g., with no stripe or CT stripe pattern) were homozygous for the wsbin6-11 marker (420 bp) derived 'AM'. These results indicated that wsbin6-11 would be a useful marker in watermelon breeding programs aiming to select for the JT stripe pattern from other various foreground and background rind patterns.
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