Cell-Free Hydrogel System Based on a Tissue-Specific Extracellular Matrix for In Situ Adipose Tissue Regeneration
- Authors
- Kim, Jun Sung; Choi, Ji Suk; Cho, Yong Woo
- Issue Date
- Mar-2017
- Publisher
- AMER CHEMICAL SOC
- Keywords
- in situ adipose tissue regeneration; cell-free scaffold system; adipose-derived soluble extracellular matrix; thermosensitive extracellular matrix hydrogel; host cell recruitment
- Citation
- ACS APPLIED MATERIALS & INTERFACES, v.9, no.10, pp.8581 - 8588
- Indexed
- SCIE
SCOPUS
- Journal Title
- ACS APPLIED MATERIALS & INTERFACES
- Volume
- 9
- Number
- 10
- Start Page
- 8581
- End Page
- 8588
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/10090
- DOI
- 10.1021/acsami.6b16783
- ISSN
- 1944-8244
- Abstract
- Well-designed scaffolds provide appropriate niches that can effectively recruit host cells and induce differentiation of recruited cells into the desired cell types, facilitating in situ tissue regeneration. Here we report a tissue-specific extracellular matrix (ECM) hydrogel composed of adipose-derived soluble ECM (sECM) and methylcellulose (MC) as a cell-free scaffold system for adipose tissue regeneration. The sECM MC hydrogels showed a thermosensitive sol-gel phase transition and rapidly formed a soft hydrogel with a stiffness of 3.8 kPa at body temperature. An in vivo study showed that the sECM MC hydrogel facilitated the infiltration of host cell populations, particularly adipose-derived stem cells (ASCs) and adipose tissue macrophages (ATMs) that directly contribute to the adipose tissue regeneration. Moreover, the hydrogel significantly enhanced host-derived adipogenesis and angiogenesis without exogenous cells or bioactive molecules. Our results indicate that the sECM MC hydrogels provide mechanical and biochemical cues for host-derived adipose regeneration. Overall, the sECM MC hydrogels are a highly promising cell-free therapeutic approach for in situ adipose tissue regeneration.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF MATERIALS SCIENCE AND CHEMICAL ENGINEERING > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.