A Chromo-Fluorogenic Naphthoquinolinedione-Based Probe for Dual Detection of Cu2+ and Its Use for Various Water Samplesopen access
- Authors
- Kumar, Ashwani; Kumar, Subodh; Chae, Pil Seok
- Issue Date
- Feb-2022
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- fluorescence; bis(triazole)-conjugated naphthoquinolinedione; Cu2+ sensing; bio-imaging; colorimetric; ratio-metric
- Citation
- Molecules, v.27, no.3, pp 1 - 15
- Pages
- 15
- Indexed
- SCIE
SCOPUS
- Journal Title
- Molecules
- Volume
- 27
- Number
- 3
- Start Page
- 1
- End Page
- 15
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/107961
- DOI
- 10.3390/molecules27030785
- ISSN
- 1420-3049
1420-3049
- Abstract
- The presence of an abnormal amount of Cu2+ in the human body causes various health issues. In the current study, we synthesized a new naphthoquinolinedione-based probe (probe 1) to monitor Cu2+ in different water systems, such as tap water, lakes, and drain water. Two triazole units were introduced into the probe via a click reaction to increase the binding affinity to a metal ion. In day-light, probe 1 dissolved in a mixed solvent system (HEPES: EtOH = 1:4) showed a vivid color change from light greenish-yellow to pink in the presence of only Cu2+ among various metal ions. In addition, the green luminescence and fluorescence emission of the probe were effectively bleached out immediately after Cu2+ addition. The limit of detection (LOD) of the probe was 0.5 mu M when a ratio-metric method was used for metal ion detection. The fluorescence titration data of the probe with Cu2+ showed a calculated LOD of 41.5 pM. Hence, probe 1 possesses the following dual response toward Cu2+ detection: color change and fluorescence quenching. Probe 1 was also useful for detecting Cu2+ spiked in tap/lake water as well as the cytoplasm of live HeLa cells. The current system was investigated using ultraviolet-visible and fluorescence spectroscopy as well as density functional theory calculations (DFT).
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