Salvia plebeia R.Br. and Rosmarinic Acid Attenuate Dexamethasone-Induced Muscle Atrophy in C2C12 Myotubesopen access
- Authors
- Kim, Jae-Yong; Kim, Hye Mi; Kim, Ji Hoon; Guo, Shuo; Lee, Do Hyun; Lim, Gyu Min; Kim, Wondong; Kim, Chul Young
- Issue Date
- Feb-2023
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- Salvia plebeia R.Br.; Rosmarinic acid; dexamethasone; atrophy; C2C12
- Citation
- International Journal of Molecular Sciences, v.24, no.3, pp 1 - 16
- Pages
- 16
- Indexed
- SCIE
SCOPUS
- Journal Title
- International Journal of Molecular Sciences
- Volume
- 24
- Number
- 3
- Start Page
- 1
- End Page
- 16
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/112609
- DOI
- 10.3390/ijms24031876
- ISSN
- 1661-6596
1422-0067
- Abstract
- Skeletal muscle atrophy occurs when protein degradation exceeds protein synthesis and is associated with increased circulating glucocorticoid levels. Salvia plebeia R.Br. (SPR) has been used as herbal remedy for a variety of inflammatory diseases and has various biological actions such as antioxidant and anti-inflammatory activities. However, there are no reports on the effects of SPR and its bioactive components on muscle atrophy. Herein, we investigated the anti-atrophic effect of SPR and rosmarinic acid (RosA), a major compound of SPR, on dexamethasone (DEX)-induced skeletal muscle atrophy in C2C12 myotubes. Myotubes were treated with 10 mu M DEX in the presence or absence of SPR or RosA at different concentrations for 24 h and subjected to immunocytochemistry, western blot, and measurements of ROS and ATP levels. SPR and RosA increased viability and inhibited protein degradation in DEX-treated C2C12 myotubes. In addition, RosA promoted the Akt/p70S6K/mTOR pathway and reduced ROS production, and apoptosis. Furthermore, the treatment of RosA significantly recovered SOD activity, autophagy activity, mitochondrial contents, and APT levels in DEX-treated myotubes. These findings suggest that SPR and RosA may provide protective effects against DEX-induced muscle atrophy and have promising potential as a nutraceutical remedy for the treatment of muscle weakness and atrophy.
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