Mesoporous platinum nanoparticles as a peroxidase mimic for the highly sensitive determination of C-reactive protein
- Authors
- Son, Seong Eun; Gupta, Pramod K.; Hur, Won; Lee, Han Been; Park, Yosep; Park, Jiyeon; Kim, Seong Nyeon; Seong, Gi Hun
- Issue Date
- Aug-2022
- Publisher
- Springer Verlag
- Keywords
- Mesoporous nanoparticle; Nanozyme; Colorimetric detection; C-Reactive protein; Enzyme-linked immunosorbent assay
- Citation
- Analytical and Bioanalytical Chemistry, v.414, no.24, pp 7191 - 7201
- Pages
- 11
- Indexed
- SCIE
SCOPUS
- Journal Title
- Analytical and Bioanalytical Chemistry
- Volume
- 414
- Number
- 24
- Start Page
- 7191
- End Page
- 7201
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/112978
- DOI
- 10.1007/s00216-022-04271-5
- ISSN
- 1618-2642
1618-2650
- Abstract
- The generation of a mesoporous structure in platinum nanoparticles can effectively enhance physical and chemical properties. In this study, mesoporous platinum nanoparticles (MPNs) were synthesized by a soft template-mediated one-pot chemical method. To develop a mesoporous structure, Pluronic F-127 was employed. The Pluronic F-127 surfactant forms self-assembled micelles, and the micelles act as the pore-directing agents in the synthesis of nanoparticles. Scanning electron microscopy results revealed that the MPN had a uniform size of 70 nm on average and a distinct mesoporous structure. The development of a concave mesoporous structure on the surface of the MPNs can increase the surface area and facilitate the efficient transport of reactants. The synthesized MPNs exhibited peroxidase-like activity. Furthermore, the MPNs showed excellent catalytic efficiency compared to HRP, due to the high surface area derived from the presence of the mesoporous structure. The peroxidase-like MPNs were applied to the enzyme-linked immunosorbent assay (ELISA) of C-reactive protein (CRP). The MPN-based ELISA exhibited sensitive CRP detection in the range from 0.24 to 7.8 ng/mL with a detection limit of 0.13 ng/mL. Moreover, the recoveries of the CRP concentrations in spiked human serum were 98.6% and 102%. These results demonstrate that as a peroxidase mimic, the MPNs can replace the natural enzymes in conventional ELISA for sensitive CRP detection.
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