Cauliflower-like platinum nanostructures mediated photothermal and colorimetric dual-readout biosensor for sensitive cholesterol detection
- Authors
- Gupta, Pramod K.; Son, Seong Eun; Venkatesan, Jayachandran; Seong, Gi Hun
- Issue Date
- Jul-2023
- Publisher
- Elsevier BV
- Keywords
- Nanozymes; Chitosan; Platinum nanostructures; Dual -readout; Photothermal; Colorimetric; Cholesterol
- Citation
- Sensors and Actuators, B: Chemical, v.386, pp 1 - 10
- Pages
- 10
- Indexed
- SCIE
SCOPUS
- Journal Title
- Sensors and Actuators, B: Chemical
- Volume
- 386
- Start Page
- 1
- End Page
- 10
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/113312
- DOI
- 10.1016/j.snb.2023.133741
- ISSN
- 0925-4005
0925-4005
- Abstract
- Point-of-care testing has been extensively utilized for in-home diagnosis, customized testing, and pandemic monitoring. The current study aims to develop a nanozyme-mediated dual-readout (colorimetric and photo -thermal) bioassay for straightforward and sensitive detection of cholesterol. The nanozyme, cauliflower-like platinum nanostructures (CS@Pt NS) were synthesized by chemically reducing the Pt precursor in the chito-san (CS) solution. CS as a stabilizer excels at controlling the surface properties, enhancing peroxidase-like ac-tivity, and improving colloidal dispersion, and stability. The dual-readout sensor for cholesterol detection was proposed by combining cholesterol oxidase-catalyzed cholesterol oxidation with CS@Pt NS-catalyzed TMB oxidation. The oxidized TMB (TMBox) acts as a probe for both colorimetric and photothermal readouts because of having excellent absorbance in visible and near-infrared (NIR) regions. The absorption of NIR laser-driven light by the TMBox in the reaction solution generates heat, which is proportional to the concentration of cholesterol. The dual-readout sensor shows a linear detection range of 0-2 mM, with a limit of detection (LOD) of 45 mu M for the colorimetric assay and 25 mu M for the photothermal assay. Furthermore, the sensor successfully detects cholesterol in spiked human serum with acceptable accuracy.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF BIONANO ENGINEERING > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.