Apta-sensor for selective determination of dopamine using chitosan-stabilized Prussian blue nanoparticles
- Authors
- Lee, Han Been; Son, Seong Eun; Seong, Gi Hun
- Issue Date
- Aug-2023
- Publisher
- Royal Society of Chemistry
- Citation
- Journal of Materials Chemistry B, v.11, no.30, pp 1 - 11
- Pages
- 11
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Materials Chemistry B
- Volume
- 11
- Number
- 30
- Start Page
- 1
- End Page
- 11
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/113573
- DOI
- 10.1039/d3tb00799e
- ISSN
- 2050-7518
2050-750X
- Abstract
- Chitosan-stabilized Prussian blue nanoparticles (CS/PBNPs) were fabricated by a simple synthetic method and used to develop a novel aptamer-based colorimetric assay for selective determination of dopamine (DA). Scanning electron microscopy (SEM) images exhibited a uniform shape of the CS/PBNPs with an average diameter of 37.0 & PLUSMN; 3.2 nm. The CS/PBNPs exhibited strong peroxidase-like activity that catalyzed the reaction between 3,3 & PRIME;,5,5 & PRIME;-tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2). Chitosan was used for stabilization of the PBNPs and fixation of the DA aptamer on the surface of the CS/PBNPs. The catalytic mechanism of the CS/PBNPs was confirmed to involve first the decomposition of H2O2 into a hydroxyl radical (OH) and then oxidation of TMB by the OH to produce a blue color. An aptamer-based colorimetric assay was made with the CS/PBNPs to detect DA at concentrations of 0.25-100 & mu;M with a limit of detection (LOD) of 0.16 & mu;M. For comparison, a gold nanoparticle (AuNP)-based apta-sensor detected DA in concentrations of 1-25 & mu;M with a LOD of 0.55 & mu;M. The recovery results of DA concentrations (0.25, 0.5, and 1 & mu;M) from spiked human serum were 92.6%, 102.1%, and 103.9%, verifying the reliability and reproducibility of the CS/PBNP-based apta-sensor for determination of DA level in clinical applications. Moreover, compared to traditional immunoassay, this aptamer-based nanozyme activation/inhibition system needs no washing step, which is very useful to shorten the assay time and maintain high sensitivity.
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Collections - COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF BIONANO ENGINEERING > 1. Journal Articles
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