L-Cysteine-Meditated Self-Assembled PtRu Derived Bimetallic Metal–Carbon Hybrid: An Excellent Peroxidase Mimics for Colorimetric and Fluorometric Detection of Hydrogen Peroxide and Cholesterol
- Authors
- Gupta, Pramod K.; Son, Seong Eun; Seong, Gi Hun
- Issue Date
- Dec-2021
- Publisher
- John Wiley and Sons Ltd
- Keywords
- bimetallic materials; cholesterol detection; enzyme mimics; H; O-2; (2) detection; hybrid structure
- Citation
- Advanced Materials Interfaces, v.8, no.23, pp 1 - 16
- Indexed
- SCIE
SCOPUS
- Journal Title
- Advanced Materials Interfaces
- Volume
- 8
- Number
- 23
- Start Page
- 1
- End Page
- 16
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/114311
- DOI
- 10.1002/admi.202101115
- ISSN
- 2196-7350
- Abstract
- Artificial enzymes have attracted considerable attention because of their significant advantages over natural enzymes. Herein, the authors report a simple hydrothermal strategy for producing a bimetallic metal — N-, S-doped carbon (NS-C@PtRu) hybrid derived from L-cysteine-mediated self-assembly of PtRu. The peroxidase-like activity and colloidal stability of the NS-C@PtRu are tuned by varying the amount of L-cysteine, the source material for the N-, S-doped carbon. The NS-C@PtRu exhibits good dispersibility, synergistic interactions (metal–metal, metal–carbon), non-metal (N, S) doping, more catalytic active sites, improved noble metallic catalyst performance, and high utilization efficiency. As an excellent peroxidase mimic, NS-C@PtRu catalyzes the oxidation of 3,3′,5′,5′-tetramethylbenzidine (TMB), and Amplex Red (AR) substrates, producing colorimetric and fluorometric signals that offer efficient detection of H2O2. Furthermore, colorimetric and fluorometric cholesterol detection is proposed by combining cholesterol oxidase-catalyzed cholesterol oxidation with NS-C@PtRu-catalyzed TMB/AR oxidation. The fluorometric cholesterol sensor exhibited a lower limit of detection of 1.4 µmol L−1 and rapid response, whereas the colorimetric sensor exhibited a wide detection range of 0.1–6 mmol L−1 and colorimetric changes that can be seen with the naked eyes. Both sensing methods demonstrated excellent selectivity and recovery in determining cholesterol in spiked human serum samples.
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