Defined Conditions Control the Morphological Dualism of Rat Primitive Extraembryonic Endoderm Stem Cells
- Authors
- Wang, Xiaoqiong; Kim, Minjae; Jung, Kyoung Hwa; Chai, Young Gyu; Binas, Bert
- Issue Date
- Dec-2023
- Publisher
- Mary Ann Liebert Inc.
- Keywords
- chemically defined culture; EMT/MET; extraembryonic endoderm; rat; stem cells
- Citation
- Stem Cells and Development, v.32, no.23-24, pp 1 - 16
- Pages
- 16
- Indexed
- SCIE
SCOPUS
- Journal Title
- Stem Cells and Development
- Volume
- 32
- Number
- 23-24
- Start Page
- 1
- End Page
- 16
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/115955
- DOI
- 10.1089/scd.2023.0187
- ISSN
- 1547-3287
1557-8534
- Abstract
- Rat primitive extraembryonic endoderm (pXEN) stem cell lines indefinitely preserve the characteristic features of the early extraembryonic endoderm (ExEn) in vitro, but require unknown serum factors and exhibit a hybrid (mesenchymal-epithelial) phenotype. We report two chemically defined conditions that differ by the addition of the cytokine leukemia inhibitory factor (Lif) and the b-catenin-stabilizing drug Chir99021, and enable permanent self-renewal as mesenchymal and epithelial morphotypes, respectively. The morphotypes are interconvertible and equipotent, as shown by the formation of well-differentiated organoids. Surprisingly, the proliferation of both morphotypes requires Lif-type Gp130/Stat3 signaling (autocrine in the absence of added Lif) and noncanonical Wnt signaling (autocrine). In addition, the epithelial version requires b-catenin for proliferation and morphology. Interestingly, the mesenchymal cells also express key epithelial markers, but those are improperly structured and/or not functional, indicating a primed state. These results provide an improved platform for studying the proliferation and plasticity of the early ExEn, which occurs in mesenchymal and epithelial forms in vivo. © 2023 Mary Ann Liebert Inc.. All rights reserved.
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