Tailoring the mechanical stiffness of DNA nanostructures using engineered defects
- Authors
- Lee, Chanseok; Kim, Kyung Soo; Kim, Young-Joo; Lee, Jae Young; Kim, Do-Nyun
- Issue Date
- Jul-2019
- Publisher
- American Chemical Society
- Keywords
- DNA nanotechnology; Finite element analysis; Molecular dynamics simulation; Persistence length; Scaffolded DNA origami; Single-stranded DNA gap; Structural stiffness
- Citation
- ACS Nano, v.13, no.7, pp 8329 - 7336
- Pages
- -992
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- ACS Nano
- Volume
- 13
- Number
- 7
- Start Page
- 8329
- End Page
- 7336
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/118187
- DOI
- 10.1021/acsnano.9b03770
- ISSN
- 1936-0851
1936-086X
- Abstract
- As scaffolded DNA origami enables the construction of diverse DNA nanostructures with predefined shapes, precise modulation of their mechanical stiffness remains challenging. We demonstrate a modular design method to widely and precisely control the mechanical flexibility of scaffolded DNA origami nanostructures while maintaining their overall structural integrity and geometric characteristics. Individually engineered defects that are short single-stranded DNA (ssDNA) gaps could reduce up to 70% of the bending stiffness of DNA origami constructs with different cross-sectional shapes. We further developed a computational analysis platform predicting the bending stiffness of a defect-engineered DNA nanostructure quickly during the design process, to offer an efficient way of designing various DNA constructs with required mechanical stiffness in a desired shape for a targeted function. © Copyright 2019 American Chemical Society.
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