Streamlined measurement of chimeric antigen receptor T-cell concentration, size, viability and two-color phenotyping during manufacturing
- Authors
- Pajarillo, Raymone; Paruzzo, Luca; Carturan, Alberto; Ugwuanyi, Ositadimma; White, Griffin; Guruprasad, Puneeth; Ballard, Hatcher J; Patel, Ruchi P; Zhang, Yunlin; Lee, Yong Gu; Hong, Seok Jae Albert; Dittami, Gregory M.; Ruella, Marco
- Issue Date
- May-2024
- Publisher
- Taylor & Francis
- Keywords
- CAR T-cell manufacturing; cellular Immunotherapies; flow cytometry
- Citation
- Cytotherapy, v.26, no.5, pp 1 - 6
- Pages
- 6
- Indexed
- SCIE
SCOPUS
- Journal Title
- Cytotherapy
- Volume
- 26
- Number
- 5
- Start Page
- 1
- End Page
- 6
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/118344
- DOI
- 10.1016/j.jcyt.2024.01.007
- ISSN
- 1465-3249
1477-2566
- Abstract
- Background aims: The successful development of CD19-targeted chimeric antigen receptor (CAR) T-cell therapies has led to an exponential increase in the number of patients recieving treatment and the advancement of novel CAR T products. Therefore, there is a strong need to develop streamlined platforms that allow rapid, cost-effective, and accurate measurement of the key characteristics of CAR T cells during manufacturing (i.e., cell number, cell size, viability, and basic phenotype). Methods: In this study, we compared the novel benchtop cell analyzer Moxi GO II (ORFLO Technologies), which enables simultaneous evaluation of all the aforementioned parameters, with current gold standards in the field: the Multisizer Coulter Counter (cell counter) and the BD LSRFortessa (flow cytometer). Results: Our results demonstrated that the Moxi GO II can accurately measure cell number and cell size (i.e., cell volume) while simultaneously assessing simple two-color flow cytometry parameters, such as CAR T-cell viability and CD4 or CAR expression. Conclusions: These measurements are comparable with those of gold standard instruments, demonstrating that the Moxi GO II is a promising platform for quickly monitoring CAR T-cell growth and phenotype in research-grade and clinical samples. © 2024 International Society for Cell & Gene Therapy
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