Enhanced acute anti-inflammatory effects of CORM-2-loaded nanoparticles via sustained carbon monoxide delivery
- Authors
- Qureshi, Omer Salman; Zeb, Alam; Akram, Muhammad; Kim, Myung-Sic; Kang, Jong-Ho; Kim, Hoo-Seong; Majid, Arshad; Han, Inbo; Chang, Sun-Young; Bae, Ok-Nam; Kim, Jin-Ki
- Issue Date
- Nov-2016
- Publisher
- ELSEVIER
- Keywords
- Carbon monoxide; CORM-2; Lipid nanoparticles; Sustained release; Anti-inflammatory effect
- Citation
- EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, v.108, pp.187 - 195
- Indexed
- SCIE
SCOPUS
- Journal Title
- EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
- Volume
- 108
- Start Page
- 187
- End Page
- 195
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/12558
- DOI
- 10.1016/j.ejpb.2016.09.008
- ISSN
- 0939-6411
- Abstract
- The aim of this study was to enhance the anti-inflammatory effects of carbon monoxide (CO) via sustained release of CO from carbon monoxide-releasing molecule-2-loaded lipid nanoparticles (CORM-2-NPs). CORM-2-NPs were prepared by hot high pressure homogenization method using trilaurin as a solid lipid core and Tween 20/Span 20/Myrj S40 as surfactant mixture. The physicochemical properties of CORM-2-NPs were characterized and CO release from CORM-2-NPs was assessed by myoglobin assay. In vitro anti-inflammatory effects were evaluated by nitric oxide assay in lipopolysaccharide-stimulated RAW 264.7 macrophages. In vivo anti-inflammatory activity was investigated by measuring paw volumes and histological examination in carrageenan-induced rat paw edema. Spherical CORM-2-NPs were around 100 nm with narrow particle size distribution. The sustained CO release from CORM-2-NPs was observed and the half-life of CO release increased up to 10 times compared with CORM-2 solution. CORM-2-NPs showed enhanced in vitro anti-inflammatory effects by inhibition of nitric oxide production. Edema volume in rat paw was significantly reduced after treatment with CORM-2-NPs. Taken together, CORM-2-NPs have a great potential for CO therapeutics against inflammation via sustained release of CO. (C) 2016 Elsevier B.V. All rights reserved.
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