Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo
- Authors
- Yoon, Hwa In; Yhee, Ji Young; Na, Jin Hee; Lee, Sangmin; Lee, Hyukjin; Kang, Sun-Woong; Chang, Hyeyoun; Ryu, Ju Hee; Lee, Seulki; Kwon, Ick Chan; Cho, Yong Woo; Kim, Kwangmeyung
- Issue Date
- Apr-2016
- Publisher
- AMER CHEMICAL SOC
- Keywords
- STEM-CELLS; STRATEGIES; THERAPY; PROLIFERATION; NANOPARTICLES; SELECTIVITY; CARTILAGE; SURVIVAL; DISEASE
- Citation
- BIOCONJUGATE CHEMISTRY, v.27, no.4, pp.927 - 936
- Indexed
- SCIE
SCOPUS
- Journal Title
- BIOCONJUGATE CHEMISTRY
- Volume
- 27
- Number
- 4
- Start Page
- 927
- End Page
- 936
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/14124
- DOI
- 10.1021/acs.bioconjchem.6b00010
- ISSN
- 1043-1802
- Abstract
- Establishment of an appropriate cell labeling and tracking method is essential for the development of cell-based therapeutic strategies. Here, we are introducing a new method for cell labeling and tracking by combining metabolic gylcoengineering and bioorthogonal copper-free Click chemistry. First, chondrocytes were treated with tetraacetylated N-azidoacetyl-D-mannosamine (Ac(4)ManNAz) to generate unnatural azide groups (-N-3) on the surface of the cells. Subsequently, the unnatural azide groups on the cell surface were specifically conjugated with near-infrared fluorescent (NIRF) dye-tagged dibenzyl cyclooctyne (DBCO-650) through bioorthogonal copper-free Click chemistry. Importantly, DBCO-650-labeled chondrocytes presented strong NIRF signals with relatively low cytotoxicity and the amounts of azide groups and DBCO-650 could be easily controlled by feeding different amounts of Ac4ManNAz and DBCO-650 to the cell culture system. For the in vivo cell tracking, DBCO-650-labeled chondrocytes (1 x 10(6) cells) seeded on the 3D scaffold were subcutaneously implanted into mice and the transplanted DBCO-650-labeled chondrocytes could be effectively tracked in the prolonged time period of 4 weeks using NIRF imaging technology. Furthermore, this new cell labeling and tracking technology had minimal effect on cartilage formation in vivo.
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