HPLC Determination of Bioactive Flavonoids in Hovenia dulcis Fruit Extracts
- Authors
- Park, Jong Suk; Kim, In Sook; Rehman, Shaheed Ur; Na, Chun-Soo; Yoo, Hye Hyun
- Issue Date
- Feb-2016
- Publisher
- Preston Publications, Inc.
- Keywords
- SEMEN-SEU-FRUCTUS; ABSOLUTE STEREOSTRUCTURES; TRITERPENE GLYCOSIDES; ANTIOXIDANT ACTIVITY; THUNB; QUERCETIN; MYRICETIN; DIHYDROMYRICETIN; SEEDS; MICE
- Citation
- Journal of Chromatographic Science, v.54, no.2, pp.130 - 135
- Indexed
- SCIE
SCOPUS
- Journal Title
- Journal of Chromatographic Science
- Volume
- 54
- Number
- 2
- Start Page
- 130
- End Page
- 135
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/14561
- DOI
- 10.1093/chromsci/bmv114
- ISSN
- 0021-9665
- Abstract
- A simple, accurate and reproducible reversed-phase liquid chromatographic method was developed for qualitative and quantitative determination of four bioactive flavonoids (ampelopsin, taxifolin, myricetin and quercetin) from the fruit-stalk extract of Hovenia dulcis Thunb. Chromatographic separation was performed on a C18 column (4.6 x 150 mm, 3.5 mu m) with mobile phase consisting of 0.1% acetic acid and 100% acetonitrile at a flow rate of 1.0 mL/min. The analysis was performed using a diode array detector at 365 nm. The method was validated in terms of selectivity, linearity, accuracy, precision and recovery. Good linearity was observed over the investigated concentration range (10-500 mu g/mL), with correlation coefficient values greater than 0.99. The intra-and inter-day precisions over the concentration range were <3.91% (relative standard deviation), and the accuracy was between 91.57 and 106.66%. The mean recovery for all the analytes was 100.87%. This method was successfully applied in the quality assessment of bioactive flavonoids in the fruit-stalk extract of H. dulcis.
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