Proteolytic disassembly of peptide-mediated graphene oxide assemblies for turn-on fluorescence sensing of proteases
- Authors
- Yang, Jin-Kyoung; Kwak, Seon-Yeong; Jeon, Su-Ji; Lee, Eunjin; Ju, Jong-Min; Kim, Hye-In; Lee, Yoon-Sik; Kim, Jong-Ho
- Issue Date
- May-2016
- Publisher
- Royal Society of Chemistry
- Keywords
- RESONANCE ENERGY-TRANSFER; HIGHLY SENSITIVE DETECTION; MATRIX METALLOPROTEINASE-2; IN-VIVO; OPTICAL-DETECTION; CARCINOMA CELLS; QUANTUM DOTS; BIOSENSOR; SENSOR; PROBE
- Citation
- Nanoscale, v.8, no.24, pp.12272 - 12281
- Indexed
- SCIE
SCOPUS
- Journal Title
- Nanoscale
- Volume
- 8
- Number
- 24
- Start Page
- 12272
- End Page
- 12281
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/16028
- DOI
- 10.1039/c6nr02815b
- ISSN
- 2040-3364
- Abstract
- Molecule-induced assembly of nanomaterials can alter their unique chemical and physical properties, which can be a promising approach for sensing. Herein, we demonstrate an optical 'turn-on' biosensor for the detection of matrix metalloproteinase-2 (MMP-2), fabricated by means of a peptide-induced assembly of fluorescent graphene oxide (GO). Functionalization of GO with a peptide substrate for MMP-2 bearing a thiol group leads to its self-assembly via disulfide bonding, accompanied by self-quenching of GO's strong fluorescence. This peptide-induced GO assembly is then disassembled by proteolytic cleavage in the presence of MMP-2, thereby restoring the level of self-quenched GO fluorescence. With this approach, we are able to detect MMP-2 and to investigate the kinetic parameters of MMP-2 activity. The GO-peptide assembly is successfully applied to the selective and sensitive detection of MMP-2 secreted by living cells, human hepatocytes HepG2, at a concentration of 2 ng mL(-1).
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