A novel nanoprobe for the sensitive detection of Francisella tularensis
- Authors
- Kim, Ji-eun; Seo, Youngmin; Jeong, Yoon; Hwang, Mintai P.; Hwang, Jangsun; Choo, Jaeburn; Hong, Jong Wook; Jeon, Jun Ho; Rhie, Gi-eun; Choi, Jonghoon
- Issue Date
- Nov-2015
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Nanoprobes; Francisella tularensis; Apoferritin; Immunoassay; Biosensing
- Citation
- JOURNAL OF HAZARDOUS MATERIALS, v.298, pp.188 - 194
- Indexed
- SCIE
SCOPUS
- Journal Title
- JOURNAL OF HAZARDOUS MATERIALS
- Volume
- 298
- Start Page
- 188
- End Page
- 194
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/16518
- DOI
- 10.1016/j.jhazmat.2015.05.041
- ISSN
- 0304-3894
- Abstract
- Francisella tularensis is a human zoonotic pathogen and the causative agent of tularemia, a severe infectious disease. Given the extreme infectivity of F. tularensis and its potential to be used as a biological warfare agent, a fast and sensitive detection method is highly desirable. Herein, we construct a novel detection platform composed of two units: (I) Magnetic beads conjugated with multiple capturing antibodies against F. tularensis for its simple and rapid separation and (2) Genetically-engineered apoferritin protein constructs conjugated with multiple quantum dots and a detection antibody against F. tularensis for the amplification of signal. We demonstrate a 10-fold increase in the sensitivity relative to traditional lateral flow devices that utilize enzyme-based detection methods. We ultimately envision the use of our novel nanoprobe detection platform in future applications that require the highly-sensitive on-site detection of high-risk pathogens. (C) 2015 Elsevier B.V. All rights reserved.
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