Photo-crosslinkable hydrogel-based 3D microfluidic culture device
- Authors
- Lee, Youlee; Lee, Jong Min; Bae, Pan-Kee; Chung, Il Yup; Chung, Bong Hyun; Chung, Bong Geun
- Issue Date
- Apr-2015
- Publisher
- John Wiley & Sons Ltd.
- Keywords
- Hydrogel; Microfluidic device; Stem cell
- Citation
- Electrophoresis, v.36, no.7-8, pp.994 - 1001
- Indexed
- SCIE
SCOPUS
- Journal Title
- Electrophoresis
- Volume
- 36
- Number
- 7-8
- Start Page
- 994
- End Page
- 1001
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/18769
- DOI
- 10.1002/elps.201400465
- ISSN
- 0173-0835
- Abstract
- We developed the photo-crosslinkable hydrogel-based 3D microfluidic device to culture neural stem cells (NSCs) and tumors. The photo-crosslinkable gelatin methacrylate (GelMA) polymer was used as a physical barrier in the microfluidic device and collagen type I gel was employed to culture NSCs in a 3D manner. We demonstrated that the pore size was inversely proportional to concentrations of GelMA hydrogels, showing the pore sizes of 5 and 25 w/v% GelMA hydrogels were 34 and 4 m, respectively. It also revealed that the morphology of pores in 5 w/v% GelMA hydrogels was elliptical shape, whereas we observed circular-shaped pores in 25 w/v% GelMA hydrogels. To culture NSCs and tumors in the 3D microfluidic device, we investigated the molecular diffusion properties across GelMA hydrogels, indicating that 25 w/v% GelMA hydrogels inhibited the molecular diffusion for 6 days in the 3D microfluidic device. In contrast, the chemicals were diffused in 5 w/v% GelMA hydrogels. Finally, we cultured NSCs and tumors in the hydrogel-based 3D microfluidic device, showing that 53-75% NSCs differentiated into neurons, while tumors were cultured in the collagen gels. Therefore, this photo-crosslinkable hydrogel-based 3D microfluidic culture device could be a potentially powerful tool for regenerative tissue engineering applications.
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