Dose- and time-dependent expression of aryl hydrocarbon receptor (AhR) and aryl hydrocarbon receptor nuclear translocator (ARNT) in PCB-, B[a]P-, and TBT-exposed intertidal copepod Tigriopus japonicus
- Authors
- Kim, Bo-Mi; Rhee, Jae-Sung; Hwang, Un-Ki; Seo, Jung Soo; Shin, Kyung-Hoon; Lee, Jae-Seong
- Issue Date
- Feb-2015
- Publisher
- Pergamon Press Ltd.
- Keywords
- Copepod; Tigriopus japonicus; AhR; ARNT; Polychlorinated biphenyl; Benzo[a]pyrene
- Citation
- Chemosphere, v.120, pp.398 - 406
- Indexed
- SCIE
SCOPUS
- Journal Title
- Chemosphere
- Volume
- 120
- Start Page
- 398
- End Page
- 406
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/18873
- DOI
- 10.1016/j.chemosphere.2014.07.099
- ISSN
- 0045-6535
- Abstract
- The aryl hydrocarbon receptor (AhR) and aryl hydrocarbon nuclear translocator (ARNT) genes from the copepod Tigriopus japonicus (Tj) were cloned to examine their potential functions in the invertebrate putative AhR-CYP signaling pathway. The amino acid sequences encoded by the Tj-AhR and Tj-ARNT genes showed high similarity to homologs of Daphnia and Drosophila, ranging from 68% and 70% similarity for the AhR genes to 56% for the ARNT genes. To determine whether Tj-AhR and Tj-ARNT are modulated by environmental pollutants, transcriptional expression of Tj-AhR and Tj-ARNT was analyzed in response to exposure to five concentrations of polychlorinated biphenyl (PCB 126) (control, 10, 50, 100, 500 mu g L-1), benzo[a]pyrene (B[a]P) (control, 5, 10, 50, 100 mu g L-1), and tributyltin (TBT) (control, 1, 5, 10, 20 mu g L-1) 24 h after exposure. A time-course experiment (0, 3, 6, 12, 24 h) was performed to analyze mRNA expression patterns after exposure to PCB, B[a]P, and TBT. T. japonicus exhibited dose-dependent and time-dependent upregulation of Tj-AhR and Tj-ARNT in response to pollutant exposure, and the degree of expression was dependent on the pollutant, suggesting that pollutants such as PCB, B[a]P, and TBT modulate expression of Tj-AhR and Tj-ARNT genes in the putative AhR-CYP signaling pathway. (C) 2014 Elsevier Ltd. All rights reserved.
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