Synthesis, Characterization, and Directional Binding of Anisotropic Biohybrid Microparticles for Multiplexed Biosensing
- Authors
- Jung, Chan Woo; Jalani, Ghulam; Ko, Juhui; Choo, Jaebum; Lim, Dong Woo
- Issue Date
- Jan-2014
- Publisher
- WILEY-V C H VERLAG GMBH
- Keywords
- anisotropy; biohybrid microparticles; electrohydrodynamic co-jetting; multiplexed biosensing; sandwich immunocomplex
- Citation
- MACROMOLECULAR RAPID COMMUNICATIONS, v.35, no.1, pp.56 - 65
- Indexed
- SCIE
SCOPUS
- Journal Title
- MACROMOLECULAR RAPID COMMUNICATIONS
- Volume
- 35
- Number
- 1
- Start Page
- 56
- End Page
- 65
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/24134
- DOI
- 10.1002/marc.201300652
- ISSN
- 1022-1336
- Abstract
- Anisotropic microarchitectures with different physicochemical properties have been developed as advanced materials for challenging industrial and biomedical applications including switchable displays, multiplexed biosensors and bioassays, spatially-controlled drug delivery systems, and tissue engineering scaffolds. In this study, anisotropic biohybrid microparticles (MPs) spatio-selectively conjugated with two different antibodies (Abs) are first developed for fluorescence-based, multiplexed sensing of biological molecules. Poly(acrylamide-co-acrylic acid) is chemically modified with maleimide- or acetylene groups to introduce different targeting biological moieties into each compartment of anisotropic MPs. Modified polymer solutions containing two different fluorescent dyes are separately used for electrohydrodynamic co-jetting with side-by-side needle geometry. The anisotropic MPs are chemically stabilized by thermal imidization, followed by bioconjugation of two different sets of polyclonal Abs with two individual compartments via maleimide-thiol coupling reaction and Huisgen 1,3-dipolar cycloaddition. Finally, two compartments of the anisotropic biohybrid MPs are spatio-selectively associated with the respective monoclonal Ab-immobilized substrate in the presence of the antigen by sandwich-type immunocomplex formation, resulting in their ordered orientation due to the spatio-specific molecular interaction, as confirmed by confocal laser scanning microscopy. In conclusion, anisotropic biohybrid MPs capable of directional binding have great potential as a new fluorescence-based multiplexing biosensing system.
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