Simultaneous determination of bioactive phenolic compounds in the stem extract of Rhus verniciflua stokes by high performance liquid chromatography
- Authors
- Kim, Sun-A; Kim, Seung Hyun; Kim, In Sook; Lee, Dongho; Dong, Mi-Sook; Na, Chun-Soo; Nguyen Xuan Nhiem; Yoo, Hye Hyun
- Issue Date
- Dec-2013
- Publisher
- Elsevier BV
- Keywords
- Rhus verniciflua stokes; Phenolic compounds; HPLC; Validation
- Citation
- Food Chemistry, v.141, no.4, pp.3813 - 3819
- Indexed
- SCIE
SCOPUS
- Journal Title
- Food Chemistry
- Volume
- 141
- Number
- 4
- Start Page
- 3813
- End Page
- 3819
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/25943
- DOI
- 10.1016/j.foodchem.2013.06.068
- ISSN
- 0308-8146
- Abstract
- A simple, sensitive, and precise reversed-phase liquid chromatographic method was developed for the quantitative determination of 4 bioactive phenolic compounds (gallic acid, fustin, fisetin, and sulfuretin) from the stem extract of Rhus verniciflua stokes. Chromatographic analysis was performed on a Capcell Pak C-18 column (150 x 4.6 mm, 3 mu m) with a mobile phase consisting of 0.1% formic acid and 90% acetonitrile at a flow rate of 1 mL/min. Quantitation was performed using a UV-vis detector at 260 nm. The method was validated in terms of selectivity, linearity, accuracy, precision, and recovery. Excellent linear behavior was observed over the investigated concentration range (10-500 mu g/mL for gallic acid, fustin, and fisetin; 0.5-100 mu g/mL for sulfuretin) with correlation coefficient (r(2)) values >0.99. The intra- and inter-day precision over the concentration range of compounds was less than 6.65% (relative standard deviation) and the accuracy was between 92.42% and 103.62%. The mean recoveries for all the analytes were more than 92.18%. This method was successfully applied for the analysis of bioactive phenolic compounds in the R. verniciflua extracts. (C) 2013 Elsevier Ltd. All rights reserved.
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