Atomically-tailored graphene oxide displaying enhanced fluorescence for the improved optical sensing of MMP-2
- Authors
- Yang, Jin-Kyoung; Hwang, In-Jun; Jeon, Su-Ji; Ju, Jong-Min; Kim, Hye-In; Yim, DaBin; Lee, Yoon-Sik; Kim, Jong-Ho
- Issue Date
- Apr-2019
- Publisher
- Elsevier BV
- Keywords
- Graphene oxide; Heteroatom doping; MMP-2; Optical biosensor; Sonic wave-assisted reaction
- Citation
- Sensors and Actuators, B: Chemical, v.284, pp.485 - 493
- Indexed
- SCIE
SCOPUS
- Journal Title
- Sensors and Actuators, B: Chemical
- Volume
- 284
- Start Page
- 485
- End Page
- 493
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/3339
- DOI
- 10.1016/j.snb.2018.12.100
- ISSN
- 0925-4005
- Abstract
- Modulation of the optical properties of two-dimensional nanomaterials is a crucial step to improve their sensing performance for the optical detection of target molecules of interest. Here, we report a mild, simple and effective approach for heteroatoms-doping of graphene oxide (GO) without size alteration, resulting in enhanced fluorescence properties. Sulfur and nitrogen atoms were chemically doped onto the basal plane of GO (NSGO) via a sonic wave-assisted reaction with cysteine as an S and N dopant under acidic conditions at room temperature. As-prepared NSGO retained the same lateral size as pristine GO, but possessed a large proportion of thiopyran sulfur and pyrrolic nitrogen. In addition, NSGO exhibited highly enhanced fluorescence with strong resistance to the pH and polarity of solution. Mechanistic investigation suggested that S-doping was essential for the improved fluorescence properties of NSGO. Finally, to investigate the effect of heteroatoms-doping on sensing performance, fluorescent NSGO was employed as a fluorescent biosensor for the detection of matrix metalloproteinases 2 (MMP-2) via a peptide-induced assembly and self-quenching principle. As compared to pristine GO, NSGO assemblies exhibited more significant fluorescence quenching, but upon addition of MMP-2, the NSGO assemblies were disassembled, leading to the higher restoration of the quenched fluorescence, eventually resulting in the more sensitive detection of MMP-2.
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Collections - COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF MATERIALS SCIENCE AND CHEMICAL ENGINEERING > 1. Journal Articles
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