Transcriptome analysis shows ambiguous phenotypes of murine primitive endoderm-related stem cell lines
- Authors
- Zhong, Yixiang; Binas, Bert
- Issue Date
- Apr-2019
- Publisher
- WILEY
- Keywords
- extraembryonic endoderm; mice; nEnd; primitive endoderm (PrE); pXEN; XEN
- Citation
- GENES TO CELLS, v.24, no.4, pp.324 - 331
- Indexed
- SCIE
SCOPUS
- Journal Title
- GENES TO CELLS
- Volume
- 24
- Number
- 4
- Start Page
- 324
- End Page
- 331
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/3384
- DOI
- 10.1111/gtc.12678
- ISSN
- 1356-9597
- Abstract
- Primitive endoderm (PrE)-related cell lines (XEN, pXEN and nEnd cells) show key features of the PrE. By transcriptome analysis, we show: (a) Compared to embryonic stem cells, PrE-related cell lines are less in vivo like, although early nEnd cells are most similar to the PrE. (b) These cell lines show post-PrE features of parietal (XEN and pXEN cells) or visceral (nEnd cells) endoderm, likely driven by Tgf-beta and Wnt/Activin signaling, respectively. (c) pXEN and nEnd cell lines additionally show pre-PrE features. Hence, neither pXEN nor nEnd cell cultures represent a distinct in vivo entity. Rather, their properties are compatible with mixed and hybrid phenotypes. Our findings indicate that pre-PrE, PrE and early post-PrE phenotypes result from different niches, which need to be better understood to derive cell lines that distinctly represent the early stages of the extraembryonic endoderm.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > DEPARTMENT OF MOLECULAR & LIFE SCIENCE > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.