DNA ligation using a disposable microfluidic device combined with a micromixer and microchannel reactor
- Authors
- Ko, Yong-Jun; Maeng, Joon-Ho; Ahn, Yoomin; Hwang, Seung Yong
- Issue Date
- Oct-2011
- Publisher
- Elsevier BV
- Keywords
- DNA ligation; Micromixer; Micropillar; Micronozzle; Serpentine microreactor; PDMS-glass microbiochip
- Citation
- Sensors and Actuators, B: Chemical, v.157, no.2, pp.735 - 741
- Indexed
- SCIE
SCOPUS
- Journal Title
- Sensors and Actuators, B: Chemical
- Volume
- 157
- Number
- 2
- Start Page
- 735
- End Page
- 741
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/36516
- DOI
- 10.1016/j.snb.2011.05.016
- ISSN
- 0925-4005
- Abstract
- A novel PDMS and glass-based microfluidic device consisting of a micromixer and microreactor for DNA ligation is described in this article. The new passive type planar micromixer is 10.33 mm long and composed of a straight channel integrated with nozzles and pillars, and the microreactor is composed of a serpentine channel. Mixing was enhanced by convective diffusion facilitated by the nozzles and pillars. The performance of the micromixer was analytically simulated and experimentally evaluated. The micromixer showed a good mixing efficiency of 87.7% at a 500 mu L/min flow rate (Re = 66.5). DNA ligation was successfully performed using the new microfluidic device, and ligation time was shortened from 4 h to 5 min. When used for on-chip ligation, this new micromixer offers advantages of disposability and portability. (C) 2011 Elsevier B.V. All rights reserved.
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Collections - COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY > ERICA 의약생명과학과 > 1. Journal Articles
- COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF MECHANICAL ENGINEERING > 1. Journal Articles
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