Log-scale dose response of inhibitors on a chip
- Authors
- Yun, Jae-young; Jambovane, Sachin R.; Kim, Se-kwon; Cho, Sung-hak; Duin, Evert C.; Hong, Jong Wook
- Issue Date
- Aug-2011
- Publisher
- AMER CHEMICAL SOC
- Citation
- ANALYTICAL CHEMISTRY, v.83, no.16, pp.6148 - 6153
- Indexed
- SCIE
SCOPUS
- Journal Title
- ANALYTICAL CHEMISTRY
- Volume
- 83
- Number
- 16
- Start Page
- 6148
- End Page
- 6153
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/37247
- DOI
- 10.1021/ac201177g
- ISSN
- 0003-2700
- Abstract
- We demonstrate the accommodation of log-scale concentration gradients of inhibitors on a single microfluidic chip with a semidirect dilution capability of reagents for the determination of the half-inhibitory concentration or IC 50. The chip provides a unique tool for hosting a wide-range of concentration gradient for studies that require an equal distribution of measuring points on a logarithmic scale. Using Matrix metalloproteinase IX and three of its inhibitors, marimastat, batimastat, and CP471474, we evaluated the IC 50 of each inhibitor with a single experiment. The present work could be applied to the systematic study of biochemical binding and inhibition processes particularly in the field of mechanistic enzymology and the pharmaceutical industry. © 2011 American Chemical Society.
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