A Self-Assembled Monolayer-Based Micropatterned Array for Controlling Cell Adhesion and Protein Adsorption
- Authors
- Kim, Dong Jin; Lee, Jong Min; Park, Jin-Goo; Chung, Bong Geun
- Issue Date
- May-2011
- Publisher
- Wiley - V C H Verlag GmbbH & Co.
- Keywords
- surface micropatterning; self-assembled monolayer; cell adhesion; protein adsorption; wettability
- Citation
- Biotechnology and Bioengineering, v.108, no.5, pp.1194 - 1202
- Indexed
- SCIE
SCOPUS
- Journal Title
- Biotechnology and Bioengineering
- Volume
- 108
- Number
- 5
- Start Page
- 1194
- End Page
- 1202
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/38109
- DOI
- 10.1002/bit.23029
- ISSN
- 0006-3592
- Abstract
- We developed a surface micropatterning technique to control the cell adhesion and protein adsorption. This micropatterned array system was fabricated by a photolithography technique and self-assembled monolayer (SAM) deposition. It was hypothesized that the wettability and functional terminal group would regulate cell adhesion and protein adsorption. To demonstrate this hypothesis, glass-based micropatterned arrays with various functional terminal groups, such as amine (NH2) group (3-aminopropyl-triethoxysilane, APT), methyl (CH3) group (trichlorovinylsilane, TVS), and fluorocarbon (CF3) group (trichloro(1H, 1H, 2H, 2H-perfluorooctyl)silane, FOTS), were used. The contact angle was measured to determine the hydrophilic and hydrophobic properties of materials, demonstrating that TVS and FOTS were hydrophobic, whereas APTs were relatively hydrophilic. The cell adhesion was significantly affected by the wettability, showing that the cells were not adhered to hydrophobic surfaces, such as TVS and FOTS. Thus, the cells were selectively adhered to glass substrates within TVS- and FOTS-based micropatterned arrays. However, the cells were randomly adhered to APTs-based micropatterned arrays due to hydrophilic property of APTs. Furthermore, the protein adsorption of the SAM-based micropatterned array was analyzed, showing that the protein was more absorbed to the TVS surface. The surface functional terminal group enabled the control of protein adsorption. Therefore, this SAM-based micropatterned array system enabled the control of cell adhesion and protein adsorption and could be a potentially powerful tool for regulating the cell-cell interactions in a well-defined microenvironment. Biotechnol. Bioeng. 2011;108: 1194-1202. (C) 2010 Wiley Periodicals, Inc.
- Files in This Item
-
Go to Link
- Appears in
Collections - COLLEGE OF ENGINEERING SCIENCES > DEPARTMENT OF MATERIALS SCIENCE AND CHEMICAL ENGINEERING > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/38109)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.