Monitoring the gene expression profiles of doxorubicin-resistant acute myelocytic leukemia cells by DNA microarray analysis
- Authors
- Song, Ju Han; Choi, Cheol Hee; Yeom, Hye-Jung; Hwang, Seung Yong; Kim, Tae Sung
- Issue Date
- Jun-2006
- Publisher
- Elsevier BV
- Keywords
- leukemia; doxorubicin; resistance; DNA microarray
- Citation
- Life Sciences, v.79, no.2, pp.193 - 202
- Indexed
- SCIE
SCOPUS
- Journal Title
- Life Sciences
- Volume
- 79
- Number
- 2
- Start Page
- 193
- End Page
- 202
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/44831
- DOI
- 10.1016/j.lfs.2005.12.054
- ISSN
- 0024-3205
- Abstract
- Acquired drug-resistance phenotype is a key factor in the relapse of patients suffering hematological malignancies. In order to investigate the genes involved in drug resistance, a human leukemia cell line that is resistant to doxorubicin, an anthracycline anticancer agent (AML-2/DX100), was selected and its gene expression profile was analyzed using a cDNA microarray. A number of genes were differentially expressed in the AML-2/DX100 cells, compared with the wild type (AML-2/WT). Pro-apoptotic genes such as TNFSF7 and p21 (Cip1/Waf1) were significantly downregulated, whereas the IKBKB, PCNA, stathmin 1, MCM5, MMP-2 and MRP1 genes, which are involved in anti-apoptotic or cell cycle progression, were over-expressed. The AML-2/DX100 cells were also resistant to other anticancer drugs, including daunorubicin and camptothecin, and the expression levels of the differentially regulated genes such as STMN1, MMP-2 and CTSG, were constantly maintained. This suggests that the deregulated genes obtained from the DNA microarray analysis in a cell line model of drug resistance might contribute to the acquired drug resistance after chronic exposure. (c) 2006 Elsevier Inc. All rights reserved.
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