Polyethylenimine-mediated cellular uptake, nucleus trafficking and expression of cytokine plasmid DNA
- Authors
- Oh, Yukyoung; Suh, Dongchul; Kim, Jung Mogg; Choi, Hangon; Shin, K; Ko, Jaejung
- Issue Date
- Dec-2002
- Publisher
- Nature Publishing Group
- Keywords
- polyethylenimine; uptake; nucleus trafficking; cytokine; plasmid DNA
- Citation
- Gene Therapy, v.9, no.23, pp 1627 - 1632
- Pages
- 6
- Indexed
- SCIE
SCOPUS
- Journal Title
- Gene Therapy
- Volume
- 9
- Number
- 23
- Start Page
- 1627
- End Page
- 1632
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/46769
- DOI
- 10.1038/sj.gt.3301735
- ISSN
- 0969-7128
1476-5462
- Abstract
- Although polyethylenimine (PEI) has been widely used as a nonviral vector, there is little mechanistic understanding on PEI-mediated delivery. Here, we studied whether the expression of murine interleukin-2 (mIL-2) plasmids could be Improved by complexation with PEI at various N/P ratios, and whether the cellular uptake, nuclear translocation, and retention of plasmids could be affected by the N/P ratios. Compared with the naked mIL-2, PEI/mIL-2 complexes showed at least two orders of magnitude higher expression at Raw264 cells in the N/P ratio-dependent manner. PEI-mediated cellular uptake and nuclear trafficking of plasmids, quantitated by competitive polymerase chain reaction, also depended on the N/P ratios showing the highest cell and nuclear levels of plasmids at 10/1. The higher cellular levels of plasmid DNA after PEI-mediated delivery were also observed in other cell lines. Unlike naked plasmids, PEI/mIL2 complexes (N/P ratios greater than or equal to 4/1) showed prolonged cellular and nuclear retention of mIL-2 plasmids. The nuclear translocation and higher cellular level of plasmids given in PEI complexes were similarly observed by fluorescence microscopy. Moreover, PEI/mIL-2 complexes revealed high stability against DNase I, partly explaining the prolonged subcellular retention. These results indicate that the expression of plasmid mIL-2 might be highly enhanced by complexation with PEI and that such increased expression could be attributed by the higher cellular uptake, nuclear translocation and prolonged retention.
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