Isolation of primitive mouse extraembryonic endoderm (pXEN) stem cell lines
- Authors
- Zhong, Yixiang; Choi, Taewoong; Kim, Minjae; Jung, Kyoung Hwa; Chai, Young Gyu; Binas, Bert
- Issue Date
- Jul-2018
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Stem cells; Blastocyst; Mice; Rats; Extraembryonic endoderm
- Citation
- STEM CELL RESEARCH, v.30, pp.100 - 112
- Indexed
- SCIE
SCOPUS
- Journal Title
- STEM CELL RESEARCH
- Volume
- 30
- Start Page
- 100
- End Page
- 112
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/5795
- DOI
- 10.1016/j.scr.2018.05.008
- ISSN
- 1873-5061
- Abstract
- Mouse blastocysts contain the committed precursors of the extraembryonic endoderm (ExEn), which express the key transcription factor Oct4, depend on LIF/LIF-like factor-driven Jak/Stat signaling, and initially exhibit lineage plasticity. Previously described rat blastocyst-derived ExEn precursor-like cell lines (XENP cells/HypoSCs) also show these features, but equivalent mouse blastocyst-derived cell lines are lacking. We now present mouse blastocyst-derived cell lines, named primitive XEN (pXEN) cells, which share these and additional characteristics with the XENP cells/HypoSCs, but not with previously known mouse blastocyst-derived XEN cell lines. Otherwise, pXEN cells are highly similar to XEN cells by morphology, lineage-intrinsic differentiation potential, and multi-gene expression profile, although the pXEN cell profile correlates better with the blastocyst stage. Finally, we show that pXEN cells easily convert into XEN-like cells but not vice versa. The findings indicate that (i) pXEN cells are more representative than XEN cells of the blastocyst stage; (ii) mouse pXEN, rather than XEN, cells are homologs of rat XENP cells/HypoSCs, which we propose to call rat pXEN cells.
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