Cell-based electrochemical cytosensor for rapid and sensitive evaluation of the anticancer effects of saponin on human malignant melanoma cells
- Authors
- Hur, Won; Son, Seong-eun; Kim, Seongnyeon; Seong, Gi-hoon
- Issue Date
- Aug-2021
- Publisher
- Elsevier B.V.
- Keywords
- Anticancer drug assessment; Cell-based biosensor; Cytosensor; Electrochemical detection; Human skin malignant melanoma; Saponin
- Citation
- Bioelectrochemistry, v.140, pp 1 - 9
- Pages
- 9
- Indexed
- SCIE
SCOPUS
- Journal Title
- Bioelectrochemistry
- Volume
- 140
- Start Page
- 1
- End Page
- 9
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/614
- DOI
- 10.1016/j.bioelechem.2021.107813
- ISSN
- 1567-5394
1878-562X
- Abstract
- Discovering new anticancer agents and analyzing their activities is a vital part of drug development, but it requires a huge amount of time and resources, leading to the increasing demands for more-effective techniques. Herein, a novel and simple cell-based electrochemical biosensor, referred to as a cytosensor, was proposed to investigate the electrochemical behavior of human skin malignant melanoma (SK-MEL28) cells and the anticancer effect of saponin on cell viability. To enhance both electrocatalytic properties and biocompatibility, gold nanoparticles were electrochemically deposited onto a conductive substrate, and poly-L-lysine was further added to the electrode surface. Electric signals from SK-MEL28 cells on the electrodes were obtained from cyclic voltammetry and differential pulse voltammetry. The cathodic peak current was proportional to the cell viability and showed a detection range of 2,880–40,000 cells per device with an excellent linear cell number-intensity relationship (R2= 0.9952). Furthermore, the anticancer effect of saponin on SK-MEL28 cells was clearly established at concentrations higher than 20 μM, which was highly consistent with conventional assays. Moreover, the developed electrochemical cytosensor for evaluating anticancer effects enabled rapid (<2 min), sensitive (LOQ: 2,880 cells/device), and non-invasive measurements, thus providing a new avenue for assessing the anticancer drugs in vitro. © 2021 Elsevier B.V.
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