Engineering oxygen nanobubbles for the effective reversal of hypoxia
- Authors
- Khan, Muhammad Saad; Hwang, Jangsun; Seo, Youngmin; Shin, Kyusoon; Lee, Kyungwoo; Park, Chanhwi; Choi, Yonghyun; Hong, Jong Wook; Choi, Jonghoon
- Issue Date
- Nov-2018
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Hypoxia; oxygen nanobubbles; HIF-1 alpha; MDA-MB231
- Citation
- ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, v.46, no.3, pp.S318 - S327
- Indexed
- SCIE
SCOPUS
- Journal Title
- ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
- Volume
- 46
- Number
- 3
- Start Page
- S318
- End Page
- S327
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/8050
- DOI
- 10.1080/21691401.2018.1492420
- ISSN
- 2169-1401
- Abstract
- Hypoxia, which results from an inadequate supply of oxygen, is a major cause of concern in cancer therapy as it is associated with a reduction in the effectiveness of chemotherapy and radiotherapy in cancer treatment. Overexpression and stabilization of hypoxia-inducible factor 1 alpha (HIF-1 alpha) protein in tumours, due to hypoxia, results in poor prognosis and increased patient mortality. To increase oxygen tension in hypoxic areas, micro- and nanobubbles have been investigated by various researchers. In the present research, lipid-shelled oxygen nanobubbles (ONBs) were synthesized through a sonication method to reverse hypoxic conditions created in a custom-made hypoxic chamber. Release of oxygen gas from ONBs in deoxygenated water was evaluated by measuring dissolved oxygen. Hypoxic conditions were evaluated by performing in vitro experiments on MDA-MB231 breast cancer cells through the expression of HIF-1 alpha and the fluorescence of image-iT (TM) hypoxia reagent. The results indicated the degradation of HIF-1 alpha after the introduction of ONBs. We propose that ONBs are successful in reversing hypoxia, downregulating HIF-1 alpha, and improving cellular conditions, leading to further medical applications.
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