Pre-elafin is Involved in Ultraviolet-induced Keratinocyte Apoptosis via Pro-caspase-3 Activation Associated with Cystatin-A Downregulationopen access
- Authors
- Kim, Hyangmi; Noh, Minsoo; Bae, Ok-Nam; Lee, Chang-Hoon; Lee, Ai-Young
- Issue Date
- May-2017
- Publisher
- ACTA DERMATO-VENEREOLOGICA
- Keywords
- UV; keratinocyte apoptosis; pre-elafin; cellular location; cystatin-A; pro-caspase-3
- Citation
- ACTA DERMATO-VENEREOLOGICA, v.97, no.5, pp.578 - 585
- Indexed
- SCIE
SCOPUS
- Journal Title
- ACTA DERMATO-VENEREOLOGICA
- Volume
- 97
- Number
- 5
- Start Page
- 578
- End Page
- 585
- URI
- https://scholarworks.bwise.kr/erica/handle/2021.sw.erica/9641
- DOI
- 10.2340/00015555-2621
- ISSN
- 0001-5555
- Abstract
- Pre-elafin controls keratinocyte integrity via cornified envelope formation and inhibition of desquamation, but its role in ultraviolet (UV)-induced keratinocyte apoptosis is unknown. This study examined the role of pre-elafin in volunteer skin samples and primary cultured normal human keratinocytes irradiated with phototoxic doses of UVA/narrow-band UVB, and in keratinocytes with pre-elafin overexpression/knockdown, under conditions of low and high calcium. Phototoxic doses of UV increased pre-elafin mRNA and protein expression in inverse proportion to keratinocyte survival. Pre-elafin overexpression under conditions of low calcium, which, in contrast to conditions of high calcium, was localized to the cytoplasm, increased keratinocyte apoptosis, whereas knockdown inhibited UV-induced apoptosis. Pre-elafin was co-localized with, but not bound to, cleaved caspase-3. Pre-elafin reduced cystatin-A expression, which was bound to pro-caspase-3. In conclusion, UV phototoxicity-induced pre-elafin inside keratinocytes prior to cornified envelope formation could be involved in UV-induced keratinocyte apoptosis via cystatin-A downregulation resulting in pro-caspase-3 activation.
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