In vivo COX-2 modulation and metabolite profiling of Pandanus tectorius leaves extracts
- Authors
- del Mundo, Crisfel R.; Castillo, Agnes L.; An, Seong Soo A.; Tan, Mario A.
- Issue Date
- Feb-2020
- Publisher
- SPRINGER HEIDELBERG
- Keywords
- Pandanus tectorius; Cyclooxygenase; Inflammation; Immunohistochemistry
- Citation
- 3 BIOTECH, v.10, no.3
- Journal Title
- 3 BIOTECH
- Volume
- 10
- Number
- 3
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/26103
- DOI
- 10.1007/s13205-020-2087-8
- ISSN
- 2190-572X
- Abstract
- The Pandanus tectorius methanolic (PTM) and P. tectorius aqueous (PTA) extracts were investigated for their potential in vivo anti-inflammatory activity using carrageenan-induced rat paw edema. Parameters including paw thickness measurement, histopathological, and immunohistochemical cyclooxygenase (COX)-2 expression analyses were measured and analyzed. PTA at 500 mg/kg significantly reduced inflammation after induction of carrageenan (Carr), with mean paw thickness change of 0.110 +/- 0.024 mm at sixth hour post-induction and histopathological mean inflammatory grade of 1.80 +/- 0.20. The reduced immunohistochemical COX-2 expression using PTA at 500 mg/kg was determined with mean final 3,3 '-diaminobenzidine (DAB) intensity of 63.70 +/- 2.08. The profiling of metabolites by liquid chromatography-mass spectrometry (LC-MS) revealed presence of ethyl caffeate and dihydroconiferyl alcohol as putative secondary metabolites of PTA which were the major peaks and have been reported to possess anti-inflammatory activities. This research has provided scientific insights of utilizing P. tectorius as a potential anti-inflammatory agent containing secondary metabolites which may be pharmacologically relevant.
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- 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
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