Decreased plasma α-synuclein in idiopathic Parkinson’s disease patients after adjusting hemolysis factor
- Authors
- Shim, K.H.; Kim, S.C.; Youn, Y.C.; Sung, Y.-H.; An, S.S.A.
- Issue Date
- Nov-2020
- Publisher
- Springer Verlag
- Keywords
- Hemolysis; Parkinson’s disease; α-Synuclein
- Citation
- Molecular and Cellular Toxicology, v.16, no.4, pp.477 - 484
- Journal Title
- Molecular and Cellular Toxicology
- Volume
- 16
- Number
- 4
- Start Page
- 477
- End Page
- 484
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78298
- DOI
- 10.1007/s13273-020-00104-7
- ISSN
- 1738-642X
- Abstract
- Background: Lewy bodies are pathological hallmarks for the diagnosis of Parkinson’s disease, where the core components are composed of aggregated forms of α-synuclein (α-syn). Although α-syn has been investigated as a potential biomarker for PD, its usage has been limited and still remains controversial. Objective: An accurate enzyme-linked immunosorbent assay (ELISA) was developed for the detection of α-syn in plasma. The hemolysis score was calculated to eliminate the additive α-syn levels from RBCs. Human plasma samples were collected in heparinized blood tubes from idiopathic Parkinson disease (IPD) and healthy control (HC). Result: Hemolysis score had a strong correlation with the level of plasma α-syn. From the limited set of samples in this preliminary study, decreased α-syn concentrations were observed in patients with IPD in comparison to HC after adjusting for hemolysis factor. Similar results with a commercial ELISA kit were found for measuring α-syn from the same set of samples with lower correlation and the reduced accuracy of diagnosis than the current study. Conclusion: The adjustments for the hemolysis factor would be indispensable, supporting plasma α-syn as a potential surrogate biomarker for distinguishing IPD from HC. © 2020, The Korean Society of Toxicogenomics and Toxicoproteomics 2020.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
- 의과대학 > 의학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/78298)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.