Antiskin Aging Effects of Indole Alkaloid N-Glycoside from Ginkgo Fruit (Ginkgo biloba fruit) on TNF-alpha-Exposed Human Dermal Fibroblasts
- Authors
- Choi, Yea Jung; Alishir, Akida; Jang, Taesu; Kang, Ki Sung; Lee, Sullim; Kim, Ki Hyun
- Issue Date
- Oct-2022
- Publisher
- AMER CHEMICAL SOC
- Keywords
- ginkgo fruitsi; indole alkaloid N-glycoside; antiskin aging; TNF-?; human dermal fibroblasts
- Citation
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.70, no.42, pp.13651 - 13660
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Volume
- 70
- Number
- 42
- Start Page
- 13651
- End Page
- 13660
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/86537
- DOI
- 10.1021/acs.jafc.2c05769
- ISSN
- 0021-8561
- Abstract
- Human skin aging has internal and external factors, both of which are characterized by TNF-alpha overproduction. Therefore, we aimed to identify a natural product that suppresses the damage that occurs in cutaneous dermal fibroblasts exposed to TNF-alpha. The protective effects of the indole alkaloid N-glycoside, ginkgoside B dimethyl ester (GBDE), isolated from ginkgo fruit (Ginkgo biloba fruit) were evaluated in TNF-alpha stimulated human dermal fibroblasts (HDFs). GBDE inhibited TNF-alpha-induced MMP-1 expression to 2.2 +/- 0.1-fold (p < 0.01) and reversed the decrease in collagen levels to 0.4 +/- 0.00-fold (p < 0.01) at 50 mu M. The effect of GBDE was due to the suppression of the phospolylaton of MAPKs (ERK, 0.47 +/- 0.05; JNK, 1.21 +/- 0.07; p38, 0.77 +/- 0.07-folds, p < 0.001) and Akt (0.14 +/- 0.03-fold, p < 0.001) compared to the TNF-alpha group. GBDE also reduced the expression of COX-2 to 2.06 +/- 0.12-fold (p < 0.001) and increased the expression of HO-1 to 10.64 +/- 0.2-fold (p < 0.001). In addition, GBDE inhibited the expression of the pro-inflammatory cytokines (IL-8, 2.2 +/- 0.0; IL-1 beta, 1.6 +/- 0.0; IL-6, 2.0 +/- 0.10-folds, p < 0.05). These results provide experimental evidence that GBDE can protect against skin damage, including aging.
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