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Fast Protein Liquid Chromatography를 이용한 황색포도상구균 장독소 B 분리Separation of Staphylococcal Enterotoxin B by Fast Protein Liquid Chromatography
- Other Titles
- Separation of Staphylococcal Enterotoxin B by Fast Protein Liquid Chromatography
- Issue Date
- May-2015
- Publisher
- 한국산업식품공학회
- Keywords
- staphylococcal enterotoxin B (SEB); fast protein liquid chromatography (FPLC); separation; purification; detection
- Citation
- 산업식품공학, v.19, no.2, pp.111 - 116
- Indexed
- KCI
- Journal Title
- 산업식품공학
- Volume
- 19
- Number
- 2
- Start Page
- 111
- End Page
- 116
- ISSN
- 1226-4768
- Abstract
- The aim of this study is to develop a method to separate and purify the Staphylococcal enterotoxin B (SEB). The method was based on fast protein liquid chromatography (FPLC) along with gel filtration (GF) after cation exchange chromatography (CIEX) treatment. Various columns such as CM sepharose HP, Cellufine C-500, WorkBeads 40S for CIEX, and GF on Sephacryl S-100 for GF were compared in terms of separation efficiency. Consequently, Work- Beads 40S along with Sephacryl S-100 produced best separation performance. Separated SEB with developed method was verified with ELISA assay. Purified SEB with the method can be used to manufacture rapid detection kit or antibodies.
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Collections - 서울 생활과학대학 > 서울 식품영양학과 > 1. Journal Articles
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Related Researcher
- Om, Ae Son
- COLLEGE OF HUMAN ECOLOGY (DEPARTMENT OF FOOD & NUTRITION)