Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E-2 Generation in LPS-stimulated RAW264.7 Cells
- Authors
- Jung, Yu Yeon; Hong, Jin Tae; Han, Sang Bae; Park, Young Hyun; Son, Dong Ju
- Issue Date
- Dec-2015
- Publisher
- 대한면역학회
- Keywords
- Ixeris dentate Nakai; Inflammation; Nitric oxide; Prostaglandin E2; iNOS; COX-2
- Citation
- Immune Network, v.15, no.6, pp 325 - 330
- Pages
- 6
- Journal Title
- Immune Network
- Volume
- 15
- Number
- 6
- Start Page
- 325
- End Page
- 330
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/10109
- DOI
- 10.4110/in.2015.15.6.325
- ISSN
- 1598-2629
2092-6685
- Abstract
- Inflammation is the basis of severe acute and chronic diseases. This study investigated the anti-inflammatory property of a crude methanol extract (MeOH-ex) and the solvent fractions of Ixeris dentata Nakai (IDN) in LPS-stimulated murine macrophage-like cell line RAW264.7. Here, we showed that the ethyl acetate fraction (EtOAc-fr) had the most potent inhibitory activity on LPS-induced nitric oxide (NO) production among the tested samples, i.e., IDN MeOH-ex and the three different solvent fractions (chloroform, n-hexane, and EtOAc). We further found that the EtOAc-fr significantly inhibited LPS-induced prostaglandin PGE2 (PGE2) generation in RAW264.7 cells. Furthermore, the treatment with EtOAc-fr effectively suppressed the expression of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2). These results suggest that the EtOAc-fr of IDN MeOH-ex exhibits an anti-inflammatory activity in vitro by inhibiting LPS-induced NO production and PGE2 generation via suppression of iNOS and COX-2 expression.
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Collections - College of Natural Sciences > Department of Food Science and Nutrition > 1. Journal Articles
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