L-carnitine이 3T3-L1 지방세포 분화에 미치는 영향

Abstract

Purpose: This study investigates the effect of L-carnitine on 3T3-L1 cell adipocyte differentiation. Methods: The cytotoxicity of L-carnitine to 3T3-L1 was confirmed through the MTT assay, and fat accumulation of 3T3-L1 cells was observed by Oil-Red O staining. The effect of L-carnitine on adipogenesis-related protein expressions was determined with Western blot. Results: Compared to the control group, the degree of adipogenesis inhibition was confirmed by Oil-Red O staining. Intracellular liposynthesis-induced fat cell differentiation with MDI(1 μM dexamethasone, 1 μg/mL inulin, 0.5 mM 3-isobutyl-1-methylxanthine) showed inhibition rates of 86.0%, 52.3%, and 32.5% at L-carnitine concentrations of 1.25, 2.5, and 5 nM, respectively. Protein expression levels of PPARγ and C/EBPα, which are transcription factors expressed during adipocyte differentiation, decreased in a concentration-dependent manner after exposure to L-carnitine. Moreover, compared to the MDI alone treatment group, we confirmed that the expression of the fatty acid synthase regulator (FAS) decreased to 84.4%, 50.9%, and 9.3% in the L-carnitine 1.25, 2.5, and 5 nM treatment groups, respectively. Conclusion: These results indicate that L-carnitine has the potential to suppress fat accumulation and exert an anti-obesity effect.