L-carnitine이 3T3-L1 지방세포 분화에 미치는 영향Effect of L-carnitine on 3T3-L1 adipocyte differentiation
- Other Titles
- Effect of L-carnitine on 3T3-L1 adipocyte differentiation
- Authors
- 이경애; 조선기; 김성호; 이윤진
- Issue Date
- Jun-2023
- Publisher
- 한국식품조리과학회
- Keywords
- Key words: L-carnitine; 3T3-L1; adipocyte; differentiation
- Citation
- 한국식품조리과학회지, v.39, no.3, pp 227 - 233
- Pages
- 7
- Journal Title
- 한국식품조리과학회지
- Volume
- 39
- Number
- 3
- Start Page
- 227
- End Page
- 233
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/25713
- ISSN
- 2287-1780
2287-1772
- Abstract
- Purpose: This study investigates the effect of L-carnitine on 3T3-L1 cell adipocyte differentiation. Methods: The cytotoxicity of L-carnitine to 3T3-L1 was confirmed through the MTT assay, and fat accumulation of 3T3-L1 cells was observed by Oil-Red O staining. The effect of L-carnitine on adipogenesis-related protein expressions was determined with Western blot. Results: Compared to the control group, the degree of adipogenesis inhibition was confirmed by Oil-Red O staining. Intracellular liposynthesis-induced fat cell differentiation with MDI(1 μM dexamethasone, 1 μg/mL inulin, 0.5 mM 3-isobutyl-1-methylxanthine) showed inhibition rates of 86.0%, 52.3%, and 32.5% at L-carnitine concentrations of 1.25, 2.5, and 5 nM, respectively. Protein expression levels of PPARγ and C/EBPα, which are transcription factors expressed during adipocyte differentiation, decreased in a concentration-dependent manner after exposure to L-carnitine. Moreover, compared to the MDI alone treatment group, we confirmed that the expression of the fatty acid synthase regulator (FAS) decreased to 84.4%, 50.9%, and 9.3% in the L-carnitine 1.25, 2.5, and 5 nM treatment groups, respectively.
Conclusion: These results indicate that L-carnitine has the potential to suppress fat accumulation and exert an anti-obesity effect.
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Collections - College of Medicine > Department of Biochemistry > 1. Journal Articles
- College of Natural Sciences > Department of Chemistry > 1. Journal Articles
- College of Natural Sciences > Department of Food Science and Nutrition > 1. Journal Articles
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