Ethyl Acetate와 Methanol을 이용한 블루베리 추출물 대사체 분석Metabolomic analysis of ethyl acetate and methanol extracts of blueberry
- Authors
- Jo, Young-Hee; Kim, Sugyeong; Kwon, Da-Ae; Lee, Hong Jin; Choi, Hyung-Kyoon; Auh, Joong-Hyuck
- Issue Date
- Mar-2014
- Publisher
- Korean Society of Food Science and Nutrition
- Keywords
- Blueberry; LC-MS/MS; Metabolites profiling
- Citation
- Journal of the Korean Society of Food Science and Nutrition, v.43, no.3, pp 419 - 424
- Pages
- 6
- Journal Title
- Journal of the Korean Society of Food Science and Nutrition
- Volume
- 43
- Number
- 3
- Start Page
- 419
- End Page
- 424
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/13848
- DOI
- 10.3746/jkfn.2014.43.3.419
- ISSN
- 1226-3311
2288-5978
- Abstract
- 본 연구에서는 LC-MS/MS를 이용한 블루베리의 methanol과 ethyl acetate 추출 분획에 존재하는 대사체의 분석을 통해 효율적인 대사체 profiling의 가능성을 탐색하였다. LC-MS/MS에서 검출되는 대사체를 통계 처리한 결과, methanol 추출 분획에서는 5-O-feruloylquinic acid, malvidin hexoside, malvidin-3-arabinoside, petunidin-3-arabinoside, delphinidin hexoside, delphinidin, petunidin hexoside와 같은 안토시아닌 계열의 화합물들이 존재하였고, ethyl acetate 분획에서는 chlorogenic acid, chlorogenic acid dimer, 6,8-di-C-arabinopyranosylluteolin, luteolin과 같은 플라보노이드 계열의 화합물이 검출되었다. 본 연구는 기존 연구와 달리 대사체학 기법을 이용한 블루베리 추출물 전체 대사물질의 profiling을 시도한 최초의 연구로서 블루베리에 함유된 유용 성분의 스크리닝등 향후 응용 연구에 유용한 기반으로 이용될 수 있을 것으로 기대된다.
Metabolite profiling of blueberry (cultivar Spartan) was performed by extraction using different solvents, methanol and ethyl acetate, through metabolomic analysis using LC-MS/MS. Unsupervised classification method (PCA) and supervised prediction model (OPLS-DA) provided good categorization of metabolites according to the extraction solvents. Metabolites of the anthocyanin family, including delphinidin hexoside, delphinidin, 5-O-feruloylquinic acid, malvidin hexoside, malvidin-3-arabinoside, petunidin-3-arabinoside, and petunidin hexoside, were mainly detected in methanol fractions, whereas those of the flavonoid family, including chlorogenic acid, chlorogenic acid dimer, 6,8-di-C-arabinopyranosyl-luteolin, and luteolin were successfully prepared in the ethyl acetate fraction. Thus, metabolomic analysis of blueberry extracts allows for the simple profiling of whole and distinctive metabolites for future applications.
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