Metabolomic Screening of Anti-Inflammatory Compounds from the Leaves of Actinidia arguta (Hardy Kiwi)open access
- Authors
- Kim, Gyoung-Deuck; Lee, Jin Young; Auh, Joong-Hyuck
- Issue Date
- Feb-2019
- Publisher
- MDPI
- Keywords
- Anti-inflammatory; Actinidia arguta; caffeic acid derivatives; metabolomic screening
- Citation
- FOODS, v.8, no.2
- Journal Title
- FOODS
- Volume
- 8
- Number
- 2
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/18292
- DOI
- 10.3390/foods8020047
- ISSN
- 2304-8158
- Abstract
- The metabolomic screening of potential anti-inflammatory compounds in the leaves of Actinidia arguta was performed by using LC-MS/MS. Ethanol extracts were prepared, and the anti-inflammatory effects were investigated based on nitric oxide (NO) synthesis and inducible nitric oxide synthase expression in lipopolysaccharide-induced RAW 264.7 macrophages. The 75% ethanol extract showed the highest inhibitory effect on nitric oxide (NO) production, and it was further separated by in vitro bioassay-guided fractionation using preparative LC with reversed-phase column separation. Through multiple steps of fractionation, sub-fraction 1-3 was finally purified, and caffeic acid derivatives, such as caffeoylthreonic acid and danshensu (salvianic acid A), were successfully identified as key anti-inflammatory compounds by LC-MS/MS and metabolomics analyses. This is the first study identifying anti-inflammatory compounds in A. arguta (Actinidia arguta) leaves through bioassay-guided fractionation and metabolomics screening. Results of this study would be useful for the application of A. arguta leaves as a nutraceutical.
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