Apoptotic Effect of Quercetin on HT-29 Colon Cancer Cells via the AMPK Signaling Pathway
- Authors
- Kim, Hyeong-Jin; Kim, Sang-Ki; Kim, Byeong-Soo; Lee, Seung-Ho; Park, Young-Seok; Park, Byung-Kwon; Kim, So-Jung; Kim, Jin; Choi, Changsun; Kim, Jong-Suk; Cho, Sung-Dae; Jung, Ji-Won; Roh, Kyong-Hwan; Kang, Kyung-Sun; Jung, Ji-Youn
- Issue Date
- Aug-2010
- Publisher
- AMER CHEMICAL SOC
- Keywords
- Quercetin; HT-29; AMPK; p53; p21; apoptosis; cancer; chemoprevention
- Citation
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, v.58, no.15, pp 8643 - 8650
- Pages
- 8
- Journal Title
- JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
- Volume
- 58
- Number
- 15
- Start Page
- 8643
- End Page
- 8650
- URI
- https://scholarworks.bwise.kr/cau/handle/2019.sw.cau/22265
- DOI
- 10.1021/jf101510z
- ISSN
- 0021-8561
1520-5118
- Abstract
- Activation of AMP-activated protein kinase (AMPK), a physiological cellular energy sensor, strongly suppresses cell proliferation in both nonmalignant and tumor cells. This study demonstrates the mechanism of quercetin-induced apoptosis in HT-29 colon cancer cells. Treatment of cells with quercetin significantly decreased cell viability in a dose-dependent manner. Notably, quercetin increased cell cycle arrest in the G1 phase and up-regulated apoptosis-related proteins, such as AMPK, p53, and p21, within 48 h. Furthermore, in vivo experiments showed that quercetin treatment resulted in a significant reduction in tumor volume over 6 weeks, and apoptosis-related protein induction by quercetin was significantly higher in the 100 mg/kg treated group compared to the control group. All of these results indicate that quercetin induces apoptosis via AMPK activation and p53-dependent apoptotic cell death in HT-29 colon cancer cells and that it may be a potential chemopreventive or therapeutic agent against HT-29 colon cancer.
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